Ratiometric probes for hydrogencarbonate analysis in intracellular or extracellular environments using europium luminescence
Ratiometric probes for hydrogencarbonate analysis in intracellular or extracellular environments using europium luminescence
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DOI：
10.1039/b206286k
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发表时间：
2002-01-01
影响因子：
4.9
通讯作者：
Slater, R
中科院分区：
文献类型：
--
作者：
Bretonnière, Y;Cann, MJ;Slater, R
一系列六种阳离子、两性离子和阴离子铕络合物已被用于分析细胞内和细胞外范围内的碳酸氢盐浓度；包含三个戊二酸残基和敏化吖啶酮发色团 (lambda(exc) = 410 nm) 的阴离子复合物在细胞裂解物培养基中，在 5 至 15 mM HCO3- 之间，618/588 nm Eu 发射带的强度比显示出 69% 的变化。
A series of six, cationic, zwitterionic and anionic Eu complexes has been examined for the analysis of hydrogen-carbonate concentration in the intracellular and extracellular ranges; an anionic complex incorporating three glutarate residues and a sensitising acridone chromophore (lambda(exc) = 410 nm) exhibits a 69% change in the intensity ratio of the 618/588 nm Eu emission bands between 5 and 15 mM HCO3- in a cell lysate medium.