Induction of neuronal type nitric oxide synthase in skeletal muscle by chronic electrical stimulation in vivo.
Induction of neuronal type nitric oxide synthase in skeletal muscle by chronic electrical stimulation in vivo.
复制标题
通过体内慢性电刺激诱导骨骼肌中的神经元型一氧化氮合酶。
DOI：
10.1152/jappl.1997.82.4.1250
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Vaghy,PL
中科院分区：
文献类型：
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作者：
Reiser,PJ;Kline,WO;Vaghy,PL
Reiser，Peter J.，William O.Kline，and Pal L.Vaghy.慢性电刺激对骨骼肌神经元型一氧化氮合酶的诱导作用.应用期刊.Physiol.82(4)：1250-1255,1997。-快抽动骨骼肌比慢抽动肌肉含有更多的神经元型一氧化氮合酶(NNOS)，因为nNOS只存在于快(类型II)肌肉纤维中。采用慢性电刺激兔胫骨前肌和趾长伸肌作为诱导快慢型纤维型转化的方法。我们已经研究了电刺激引起的肌肉收缩活动的增加是否改变了nNOS的表达，如果是的话，nNOS的表达是否降低到慢速肌肉中的水平。肌球蛋白重链亚型表达和最大缩短速度的变化表明，刺激3wk后，肌球蛋白重链亚型的表达和最大缩短速度呈现出典型的快慢纤维型转变。同时，在刺激的肌肉中，NOS的活性增加了一倍，这与免疫印迹分析显示的nNOS表达的增加有关。这些数据表明，骨骼肌中nNOS的表达受肌肉活动的调节，在表型转换的动态阶段，这种调节并不一定遵循快抽动和慢抽动的模式。
Reiser, Peter J., William O. Kline, and Pal L. Vaghy.Induction of neuronal type nitric oxide synthase in skeletal muscle by chronic electrical stimulation in vivo.J. Appl. Physiol.82(4): 1250–1255, 1997.—Fast-twitch skeletal muscles contain more neuronal-type nitric oxide synthase (nNOS) than slow-twitch muscles because nNOS is present only in fast (type II) muscle fibers. Chronic in vivo electrical stimulation of tibialis anterior and extensor digitorum longus muscles of rabbits was used as a method of inducing fast-to-slow fiber type transformation. We have studied whether an increase in muscle contractile activity induced by electrical stimulation alters nNOS expression, and if so, whether the nNOS expression decreases to the levels present in slow muscles. Changes in the expression of myosin heavy chain isoforms and maximum velocity of shortening of skinned fibers indicated characteristic fast-to-slow fiber type transformation after 3 wk of stimulation. At the same time, activity of NOS doubled in the stimulated muscles, and this correlated with an increase in the expression of nNOS shown by immunoblot analysis. These data suggest that nNOS expression in skeletal muscle is regulated by muscle activity and that this regulation does not necessarily follow the fast-twitch and slow-twitch pattern during the dynamic phase of phenotype transformation.