Harmaline-induced tremor in mice: videotape documentation and open questions about the model.
Harmaline-induced tremor in mice: videotape documentation and open questions about the model.
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DOI：
10.7916/d8h993w3
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发表时间：
2013-01-01
期刊：
影响因子：
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通讯作者：
Kuo, Sheng-Han
中科院分区：
文献类型：
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作者：
Cheng, Melody M;Tang, Guomei;Kuo, Sheng-Han
背景：harmaline诱发的啮齿动物震颤已被广泛用作特发性震颤（ET）的动物模型。然而，在已发表的文献中没有视觉记录。方法：分别给小鼠皮下注射20mg /kg盐酸汉玛林或生理盐水，并对反应进行录像。结果：小鼠的动作和体位性震颤在皮下注射丙碱后5分钟开始，约30分钟达到高峰。震颤涉及头部、躯干、尾巴和四肢，持续了大约2小时。前肢震颤为体位性或运动性震颤，与et中观察到的震颤相似。讨论：本视频片段首次提供了鼠体内盐碱诱发震颤现象的视觉记录。我们还提出了几个悬而未决的问题，关于使用盐碱诱发的震颤来模拟ET。
BACKGROUND: Harmaline-induced tremor in rodents has been extensively used as an animal model for essential tremor (ET). However, there is no visual documentation in the published literature.METHODS: We injected mice subcutaneously with either 20 mg/kg of harmaline hydrochloride or saline and then videotaped the responses.RESULTS: Action and postural tremor in the mouse began 5 minutes after subcutaneous harmaline injection and peaked at approximately 30 minutes. The tremor involved the head, trunk, tail, and four limbs and lasted for approximately 2 hours. The forelimb tremor was postural or action tremor, similar to that observed in ET.DISCUSSION: This video segment provides the first visual documentation of the phenomenology of harmaline-induced tremor in a mouse. We also raise several unanswered questions regarding the use of harmaline-induced tremor to model ET.