Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20.
Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20.
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DOI：
10.2174/1874357901711010073
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Shi J
中科院分区：
文献类型：
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作者：
Li X;Galliher-Beckley A;Wang L;Nietfeld J;Feng W;Shi J
中国人幽门螺杆菌PRRSV是2006年在中国暴发的，以各年龄段猪的高发病率和高死亡率为特征。HP-PRRSV的免疫应答从未与传统的低致病性PRRSV株相比较。在这项研究中，我们比较了中国HV株和北美RRSV株NADC20感染引起的免疫应答。感染NADC-20的猪在第9天的抗体效价显著高于感染HV-PRRSV的猪。感染HV-PRRSV后，血清和肺组织中的肿瘤坏死因子-α和IL-10水平显著升高，血清中的干扰素-α和干扰素-γ水平显著升高。流式细胞仪分析显示，与感染NADC-20的猪相比，感染HV-PRRSV的猪外周血中的NK细胞和肺中的Th/Memory、CTL和T-reg细胞的频率显著增加。本研究表明HV-PRRSV和NADC-20具有不同的免疫学特性，这些差异可能是HV-PRRSV和NADC-20致病机制不同的原因之一。
Chinese HP-PRRSV characterized by high morbidity and mortality of all ages of pigs emerged since 2006 in China. The immune response of HP-PRRSV was never compared with conventional low pathogenic PRRSV strain. In this study, we compared the immune responses elicited by a Chinese HP-PRRSV strain HV and a North American RRSV strain NADC20 infections. Pigs infected with NADC-20 showed significantly higher Ab titers than HV-PRRSV infected pigs at 9 DPI. Infection with HV-PRRSV induced a significantly higher levels of TNF-α and IL-10 in both sera and lung tissues and higher IFN-α and IFN-γ in the serum. Flow cytometry analysis showed that HV-PRRSV infected pigs generated significantly higher frequencies of NK cells in the peripheral blood and Th/memory, CTLs, and T-reg cells in the lung as compared with NADC-20 infected pigs. This study demonstrates that different immunity profiles were elicited by HV-PRRSV and NADC-20, and these differences may contribute to the distinct pathogenesis of HV-PRRSV and NADC-20.