Genes involved in the RNA interference pathway are differentially expressed during sea urchin development.
Genes involved in the RNA interference pathway are differentially expressed during sea urchin development.
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参与RNA干扰途径的基因在海胆发育过程中存在差异表达。
DOI：
10.1002/dvdy.21353
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Wessel,GaryM
中科院分区：
文献类型：
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作者：
Song,JiaL;Wessel,GaryM
RNA介导的干扰（RNAi）是一种保守的基因沉默机制，以双链RNA为信号，触发靶mRNA的序列特异性降解，导致转录后沉默和/或翻译抑制。在海胆基因组数据库中的生物信息学搜索确定了Drosha，DGCR 5，Dicer，TRBP，Exportin-5和Argonautes的同源物。定量、真实的-时间聚合酶链反应表明，所有的mRNA在卵中积累，并且在整个早期发育过程中以可变的水平积累。整体原位RNA杂交显示，RNAi沉默途径的所有重要参与者在卵母细胞和卵子中具有丰富的mRNA积累，但在整个发育过程中具有不同的空间和时间表达模式。序列分析表明，每四个Argonautes检查含有保守的残基的Piwi结构域内的RNAseH活性的重要。这项研究阐明了RNAi沉默途径中的基因具有动态表达，因此可能在生殖细胞发育和胚胎发生过程中发挥调控作用。发展动力学236：3180-3190，2007年。© 2007 Wiley利斯公司
RNA‐mediated interference (RNAi) is a conserved gene silencing mechanism that involves double‐stranded RNA as a signal to trigger the sequence‐specific degradation of target mRNA, resulting in posttranscriptional silencing and/or translational repression. Bioinformatic searches in the sea urchin genome database identified homologs of Drosha, DGCR5, Dicer, TRBP, Exportin‐5, and Argonautes. Quantitative, real‐time polymerase chain reaction indicated that all mRNA accumulate in eggs and in variable levels throughout early development. Whole‐mount in situ RNA hybridization showed that all of the important players of the RNAi silencing pathway have abundant mRNA accumulation in oocytes and eggs, but have distinct spatial and temporal expression patterns throughout development. Sequence analysis revealed that each of the four Argonautes examined contain conserved residues important for RNAseH activity within the Piwi domain. This study elucidated that genes involved in the RNAi silencing pathway have dynamic expression and, thus, may have regulatory roles during germ cell development and embryogenesis. Developmental Dynamics 236:3180–3190, 2007. © 2007 Wiley‐Liss, Inc.