INVITRO CULTURE OF DISAGGREGATED RABBIT KERATINOCYTES
INVITRO CULTURE OF DISAGGREGATED RABBIT KERATINOCYTES
复制标题
DOI：
10.1038/icb.1990.17
复制
发表时间：
1990-04-01
影响因子：
4
通讯作者：
CLUNIE, GJA
中科院分区：
文献类型：
--
作者：
BREIDAHL, AF;JUDSON, RT;CLUNIE, GJA
描述了一种用于培养解聚的兔角质形成细胞和生产适用于移植研究的免疫纯角质形成细胞汇合片的技术。通过使用配对对照来检查各种饲养层、营养物和生长激素的益处。培养的角质形成细胞片能够完整地从培养容器中取出并作为可行的自体移植物存活，从而建立了新的动物模型用于研究对培养的角质形成细胞的同种异体移植物排斥反应。讨论了使用兔模型进行此类研究的好处以及该培养技术相对于先前描述的兔角质形成细胞培养方法的优势。
A technique is described for the culture of disaggregated rabbit keratinocytes and the production of confluent sheets of immunologically pure keratinocytes suitable for use in transplantation studies. The benefits of various feeder layers, nutrients and growth hormones were examined by the use of paired controls. The ability of the cultured keratinocyte sheets to be removed from the culture vessel intact and survive as viable autografts was demonstrated, thus establishing a new animal model for the investigation of allograft rejection responses to cultured keratinocytes. The benefits of using the rabbit model for such studies and the advantages of this culture technique over a previously described method of rabbit keratinocyte cell culture are discussed.