The Possible Influence of Non-synonymous Point Mutations within the FimA Adhesin of Non-typhoidal Salmonella (NTS) Isolates in the Process of Host Adaptation.
The Possible Influence of Non-synonymous Point Mutations within the FimA Adhesin of Non-typhoidal Salmonella (NTS) Isolates in the Process of Host Adaptation.
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DOI：
10.3389/fmicb.2017.02030
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发表时间：
2017
影响因子：
5.2
通讯作者：
Vidovic S
中科院分区：
文献类型：
--
作者：
Alshalchi S;Hayer SS;An R;Munoz-Aguayo J;Flores-Figueroa C;Nguyen R;Lauer D;Olsen K;Alvarez J;Boxrud D;Cardona C;Vidovic S
非伤寒沙门氏菌（NTS）仍然是一种全球性的病原体，影响广泛的动物物种。我们分析了大量不同宿主来源的NTS分离株，包括海德堡沙门氏菌（n = 80，禽类）、S.都柏林（50，牛）、S.鼠伤寒沙门氏菌5-变种（n = 40，猪），S. 4，5，12，：i：-（n = 40，猪），S. Cerro（n = 16，牛）和S.蒙得维的亚（n = 14，牛），使用bcfC、mgtC、ssaC、invE、pefA、pepB、sopB和siiE毒力相关基因的毒力分析、生物膜产生测定、脉冲场凝胶电泳以及fimA（粘附素）和iroN（受体）基因的全长测序。我们确定了一个关键的氨基酸取代，A169（即，苏氨酸在位置169处变为丙氨酸），在FimA蛋白中改变了FimA对N-乙酰基-D-葡糖胺的配体亲和力。这一发现清楚地表明了非同义单核苷酸多态性（nsSNPs）在粘附素功能中的重要作用，这可能会影响NTS的宿主向性。该nsSNP在S. Heidelberg和S.塞罗分离株。虽然IroN受体的情况并非如此，但该受体的遗传与NTS分离株的不同宿主来源呈正相关，表明S.肠的。我们发现，pefA，一个基因编码的主要菌毛亚基，是最分离的毒力因子。与S. Heidelberg，S.鼠伤寒变种5-和S. 4，5，12，：i：-但不与其余NTS菌株。此外，我们观察到与S. Heidelberg（2.5%）和S.鼠伤寒5-变种（7.5%）和沙门氏菌（S. 4，5，12，：i：-（0%）.这项研究提供了新的见解，宿主适应的鸟类和哺乳动物的NTS菌株的基础上的细菌抗原FimA和IroN，以及宿主适应，整体遗传相关性之间的相互关系，在这些NTS菌株的毒力潜力。
Non-typhoidal Salmonella (NTS) remains a global pathogen that affects a wide range of animal species. We analyzed a large number of NTS isolates of different host origins, including Salmonella Heidelberg (n = 80, avian), S. Dublin (50, bovine), S. Typhimurium var 5- (n = 40, porcine), S. 4,5,12,:i:- (n = 40, porcine), S. Cerro (n = 16, bovine), and S. Montevideo (n = 14, bovine), using virulence profiling of the bcfC, mgtC, ssaC, invE, pefA, stn, sopB, and siiE virulence-associated genes, a biofilm production assay, pulsed field gel electrophoresis, and the full-length sequencing of the fimA (adhesin) and iroN (receptor) genes. We determined a key amino acid substitution, A169 (i.e., threonine changed to alanine at position 169), in the FimA protein that changed ligand affinity of FimA toward N-acetyl-D-glucosamine. This finding clearly indicates the important role of non-synonymous single nucleotide polymorphism (nsSNPs) in adhesin functionality that may impact the host tropism of NTS. This nsSNP was found in S. Heidelberg and S. Cerro isolates. Although this was not the case for the IroN receptor, the phylogeny of this receptor and different host origins of NTS isolates were positively correlated, suggesting existence of specific host immune selective pressures on this unique receptor in S. enterica. We found that pefA, a gene encoding major fimbrial subunit, was the most-segregative virulence factor. It was associated with S. Heidelberg, S. Typhimurium var 5- and S. 4,5,12,:i:- but not with the rest of NTS strains. Further, we observed a significantly higher frequency of non-biofilm producers among NTS strains that do not carry pefA (42.5%) compared to S. Heidelberg (2.5%) and S. Typhimurium var 5- (7.5%) and S. 4,5,12,:i:- (0%). This study provides new insights into the host adaptation of avian and mammalian NTS isolates that are based on the bacterial antigens FimA and IroN as well as the interrelationships between host adaptation, overall genetic relatedness, and virulence potential in these NTS isolates.
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影响因子：
6.7
作者：
Kisiela DI;Chattopadhyay S;Libby SJ;Karlinsey JE;Fang FC;Tchesnokova V;Kramer JJ;Beskhlebnaya V;Samadpour M;Grzymajlo K;Ugorski M;Lankau EW;Mackie RI;Clegg S;Sokurenko EV
通讯作者：
Sokurenko EV
影响因子：
3.7
作者：
An R;Alshalchi S;Breimhurst P;Munoz-Aguayo J;Flores-Figueroa C;Vidovic S
通讯作者：
Vidovic S
影响因子：
11.8
作者：
Bangtrakulnonth A;Pornreongwong S;Pulsrikarn C;Sawanpanyalert P;Hendriksen RS;Lo Fo Wong DM;Aarestrup FM
通讯作者：
Aarestrup FM
DOI：
10.1073/pnas.94.3.934
发表时间：
1997-02-04
影响因子：
11.1
作者：
Heithoff, DM;Conner, CP;Mahan, MJ
通讯作者：
Mahan, MJ
影响因子：
3.1
作者：
Kisiela, D;Sapeta, A;Ugorski, M
通讯作者：
Ugorski, M