Design of an amperometric biosensor using polypyrrole-microgel composites containing glucose oxidase
Design of an amperometric biosensor using polypyrrole-microgel composites containing glucose oxidase
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DOI：
10.1016/j.bios.2004.05.018
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发表时间：
2004-12-15
影响因子：
12.6
通讯作者：
López-Ruiz, B
中科院分区：
文献类型：
--
作者：
Retama, JR;Cabarcos, EL;López-Ruiz, B
将聚吡咯-聚苯乙烯磺酸盐(PPy)包埋在聚丙烯酰胺(PA)中，制备了一种新型的水分散材料，并将其作为酶固定化体系用于安培生物传感器。在含有葡萄糖氧化酶(GOx)和聚吡咯(PPy)的浓乳液分散相中，通过丙烯酰胺聚合，将葡萄糖氧化酶(GOx)和水分散的聚吡咯复合物包埋在聚丙烯酰胺微凝胶中。分散相的聚合提供了尺寸在3.5至7微米之间的微粒。将聚吡咯引入到聚丙烯酰胺微粒中的目的是促进酶反应中释放的电子从催化位置直接转移到铂电极表面。用四探针法测量了微粒的电导率，并通过生物传感器对葡萄糖的厌氧检测证实了这一点。因此，聚丙烯酰胺-聚吡咯(Pappy)微粒结合了聚吡咯的导电性和聚丙烯酰胺的孔径控制。考察了聚丙烯酰胺-聚吡咯的比例和交联度对生物传感器响应的影响，以及pH和酶负载等分析参数对传感器响应的影响。Pappy生物传感器不受抗坏血酸和尿酸的干扰，可用于人体血清中的定量分析。(C)2004年，爱思唯尔出版。
A new material consisting of a water-dispersed complex of polypyrrole-polystyrensulfonate (PPy) embedded in polyacrylamide (PA) has been prepared and tested as enzyme immobilizing system for its use in amperometric biosensors. Glucose oxidase (GOx) and the water-dispersed polypyrrole complex were entrapped within polyacrylamide microgels by polymerization of acrylamide in the dispersed phase of concentrated emulsions containing GOx and PPy. Polymerization of the dispersed phase provides microparticles whose size lies between 3.5 and 7 mum. The aim of incorporating polypyrrole into the polyacrylamide microparticles was to facilitate the direct transfer of the electrons released in the enzymatic reaction from the catalytic site to the platinum electrode surface. The conductivity of the microparticles was measured by a four-point probe method and confirmed by the successful anaerobic detection of glucose by the biosensor. Thus, the polyacrylamide-polypyrrole (PAPPy) microparticles combine the conductivity of polypyrrole and the pore size control of polyacrylamide. The effects of the polyacrylamide-polypyrrole ratio and cross-linking on the biosensor response have been investigated, as well as the influence of analytical parameters such as pH and enzymatic loading. The PAPPy biosensor is free of interferences arisin from ascorbic and uric acids, which allows its use for quantitative analysis in human blood serum. (C) 2004 Published by Elsevier B.V.