Cancer cell targeting and imaging with biopolymer-based nanodevices
Cancer cell targeting and imaging with biopolymer-based nanodevices
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DOI：
10.1016/j.ijpharm.2012.11.038
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发表时间：
2013-01-30
影响因子：
5.8
通讯作者：
Borbely, Janos
中科院分区：
文献类型：
--
作者：
Hajdu, Istvan;Bodnar, Magdolna;Borbely, Janos
我们在此报告了磁共振成像（MRI）活性纳米颗粒的合成、体外和体内研究，该纳米颗粒靶向叶酸受体过度表达的肿瘤细胞。由聚γ-谷氨酸和壳聚糖生物聚合物与Gd离子制备了流体动力学尺寸为50-200 nm的自组装纳米颗粒。纳米粒子具有生物相容性、无毒，并且在水介质中可稳定数月。使用共聚焦显微镜、流式细胞术和 MR 成像对 HeLa 人宫颈癌肿瘤细胞进行的体外测定表明，叶酸靶向纳米颗粒以叶酸受体依赖性方式特异性内化。体内研究证实，与 MR 图像所代表的对照动物相比，发现了相当多的纳米系统积累。弛豫测量表明纳米颗粒-Gd 复合物极大地改变了肿瘤细胞的信号强度。由于对比度增强，它们是作为各种诊断应用（包括肿瘤早期诊断）的潜在造影剂的有吸引力的候选者。 (C) 2012 Elsevier B.V. 保留所有权利。
We report here the synthesis, in vitro and in vivo investigation of magnetic resonance imaging (MRI) active nanoparticles, which target folate receptor overexpressing tumor cells. Self-assembled nanoparticles with a hydrodynamic size of 50-200 nm were prepared from poly-gamma-glutamic acid and chitosan biopolymers with Gd-ions. The nanoparticles are biocompatible, non-toxic and stable for several months in aqueous media. In vitro assays using confocal microscopy, flow cytometry and MR imaging on HeLa human cervix carcinoma tumor cells showed that folic acid targeted nanoparticles were internalized specifically in a folate receptor dependent manner. In vivo study confirmed, that, considerable accumulation of nanosystems was found compared with the control animal represented by the MR images. Relaxometry measurements demonstrated that the nanoparticle-Gd complexes drastically change the signal intensity of the tumor cells. Because of the contrast enhancement, they are attractive candidates as potential contrast agents for a variety of diagnostic applications including early diagnosis of tumors. (C) 2012 Elsevier B. V. All rights reserved.