CAPILLARY TUBE METHOD FOR COUNTING VIABLE BACTERIA
CAPILLARY TUBE METHOD FOR COUNTING VIABLE BACTERIA
复制标题
毛细管法计数活菌
作者：
T. Yanagita
为了简化活细菌细胞的计数，我们实验室开发了一种新的方法，并已使用了几年，效果令人满意。细菌悬浮液在试管中用生理盐水适当稀释，以获得每毫升1,000至10,000个细胞。将半毫升这种悬浮液与4.5毫升融化的营养充分混合，以增强强度并放大试管内形成的菌落。在室温下离开毛细管直到琼脂变硬后，将它们水平放置在37摄氏度的孵化器中24小时。每组三份毛细血管用一条纸带固定在一起，纸带上标有适当的记号。随后，在一种特殊的设备中对菌落进行计数(见图1和图2)。将毛细管一端放入计数器的水平金属管中，然后慢慢向另一端移动。
To simplify the counting of viable bacterial cells, a new method was developed in our laboratory and has been used quite satisfactorily for several years. The bacterial suspension is properly diluted with saline in a test tube to obtain 1,000 to 10,000 cells per ml. One-half ml of this suspension is mixed thoroughly with 4.5 ml of melted nutrient desirable for strength and to magnify the colonies formed inside the tube. After leaving the capillaries at room temperature until the agar hardens, they are placed horizontally into an incubator at 37 C for 24 hr. Each group of triplicate capillaries is held together with a paper band marked with proper notations. Subsequently colonies are counted in a special device (see figures 1 and 2). The capillary is put into the horizontal metal tube of the counter in one end and shifted slowly toward the other end.