Preparation and characterization of a scaffold for vascular tissue engineering by direct-assembling of collagen and cells in a cylindrical geometry
Preparation and characterization of a scaffold for vascular tissue engineering by direct-assembling of collagen and cells in a cylindrical geometry
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DOI：
10.1002/mabi.200600242
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发表时间：
2007-05-10
影响因子：
4.6
通讯作者：
Mantovani, Diego
中科院分区：
文献类型：
--
作者：
Boccafoschi, Francesca;Rajan, Navneeta;Mantovani, Diego
在过去的 25 年里，人们一直致力于开发植入人体后能保持长期通畅的血管替代品。在这项研究中，我们展示了我们在一个正在进行的项目中的最新发现，该项目旨在利用基于胶原蛋白的支架开发组织再生血管。通过直接将胶原蛋白和细胞组装成圆柱形几何形状，开发出具有足够生物学特性的胶原蛋白支架。该技术主要涉及使用通风旋转管，使光滑细胞在圆柱形胶原基质中生长。进行扫描电子显微镜（SEM）、组织学和细胞周期分析，以评估基于细胞的支架进一步成熟的生物潜力。合规性结果表明，该技术允许细胞在胶原基质中组装，从而为要处理和安装在灌注生物反应器中的结构提供足够的刚性，以进一步生长和成熟。
In the past 25 years, several efforts have been focused on developing vascular substitutes showing long-term patency when implanted in humans. In this study, we present our last findings in an ongoing project aiming to develop a tissue-regenerated blood vessel from collagen-based scaffolds. A collagen-based scaffolds with adequate biological properties was developed by directly assembling collagen and cells in a cylindrical geometry. The technique mainly involves the use of a ventilated rotating tube allowing smooth cells to grow in a cylindrical collagen matrix. Scanning electron microscopy (SEM), histology, and cell cycle analyses were carried out in order to assess the biological potential of the cell-based scaffold for further maturation. compliance results showed that this technique allows cells to assemble in the collagen matrix, thus providing enough rigidity to the structure to be handled and mounted in a perfusion bioreactor for further growth and maturation.