Transcriptomic and Metabolic Profiling Reveals 'Green Ring' and 'Red Ring' on Jujube Fruit upon Postharvest Alternaria alternata Infection.
Transcriptomic and Metabolic Profiling Reveals 'Green Ring' and 'Red Ring' on Jujube Fruit upon Postharvest Alternaria alternata Infection.
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DOI：
10.1093/pcp/pcy252
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发表时间：
2019-01
影响因子：
4.9
通讯作者：
Shuzhi Yuan;Jiaqi Yan;Meng Wang;Xinyuan Ding;Yinan Zhang;Wusun Li;Jiankang Cao;Weibo Jiang
中科院分区：
文献类型：
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作者：
Shuzhi Yuan;Jiaqi Yan;Meng Wang;Xinyuan Ding;Yinan Zhang;Wusun Li;Jiankang Cao;Weibo Jiang
链格孢菌（Alternaria alternata）是枣采后贮藏的主要威胁。水果.我们发现天然A.黑穗病菌侵染枣果后，病斑周围出现非常典型的“绿色环”和“红环”表型。通过人工接种的方法，成功地将该表型构建到了拟南芥上。此外，病原性感染被证明对表型的发作是必要的。“红环”圈被证明是预先固定的，以阻挡“绿色环”区域作为对抗病原体攻击的战场。我们监测了感染A. coli的拟南芥的“绿色环”和“红环”组织的整体转录组学谱。与模拟接种的果实和对照完整果实相比，alternata的效果更好。在“绿色环”中获得了大量差异表达基因，其次是“红色环”。与核心和外围苯丙素和木质素途径相关的转录改变，植物激素代谢在“绿色环”和“红色环”中受到A.互隔孢侵染转录组学分析和代谢变化的综合分析揭示了在防御真菌感染中，在拟南芥上的“绿色环”和“红色环”中这些生物过程的差异但微妙地协调激活。
Alternaria alternata is the major threat to postharvest storage of jujube (Ziziphus jujuba Mill.) fruit. We found that natural A. alternata infection can cause very typical phenotype of 'green ring' and 'red ring' surrounding the disease spot on the jujube fruit. The phenotype was successfully modeled and constructed on jujubes by artificial inoculation with the pathogen. Furthermore, the pathogenic infection is evidenced essential to the onset of the phenotype. The 'red ring' circle is proved to be pre-fixed to block the 'green ring' area as a battlefield combating the pathogen's attack. We monitored the global transcriptomic profiling of 'green ring' and 'red ring' tissues from jujubes infected with A. alternata, in comparison with the mock-inoculated fruit and the control intact fruit. Large amount of differentially expressed genes were obtained in 'green ring', followed by 'red ring'. Transcriptional alterations associated with the core and peripheral phenylpropanoid and lignin pathways, plant hormonal metabolisms were greatly influenced in the 'green ring' and 'red ring' by the A. alternata infection. The integrated analysis of transcriptomic profiling and metabolic changes revealed the differentially but delicately coordinated activation of these biological processes in the 'green ring' and 'red ring' on jujubes in defensing the fungal infection.