Investigation of Simian virus 40 large T antigen in 18 autopsied malignant mesothelioma patients in Japan
Investigation of Simian virus 40 large T antigen in 18 autopsied malignant mesothelioma patients in Japan
复制标题
DOI：
10.1002/jmv.20219
复制
发表时间：
2004-12-01
影响因子：
12.7
通讯作者：
Nagashima, K
中科院分区：
文献类型：
--
作者：
Jin, M;Sawa, H;Nagashima, K
据报道，猿猴病毒40(SV40)通过接种受污染的脊髓灰质炎疫苗与人类联系在一起，可能在恶性间皮瘤的病因学中起作用。然而，在日本还没有关于SV40与恶性间皮瘤的关系的报道。采用聚合酶链式反应(PCR)分析、DNA序列分析和免疫组织化学方法研究SV40与日本恶性间皮瘤患者的关系。来自日本五家医院的18例胸膜恶性间皮瘤尸检患者的石蜡包埋样本。从石蜡切片中提取DNA后，用3组不同的引物对SV40大T抗原(Tag)基因进行PCR分析和测序。用两种不同的抗SV40标签抗体对18例恶性间皮瘤标本进行SV40标签蛋白表达的免疫组织化学检测。在8例恶性间皮瘤中检测到SV40标签基因组。三对引物中只有一对在样本中成功扩增了SV40基因组，而所有的对在对照中都产生了PCR产物，这表明病毒DNA含量很低。所有标本均未见SV40标记免疫阳性染色。这项研究表明，SV40基因组存在于日本恶性间皮瘤患者的子集中，根据他们的年龄，这些患者不太可能接种过受污染的脊髓灰质炎疫苗。
It has been reported that Simian virus 40 (SV40) is linked to human beings by inoculation of contaminated poliovaccines and may have a role in the etiology of malignant mesothelioma. However, there have been no reports describing the relationship between SV40 and malignant mesothelioma in Japan. A study was undertaken to investigate whether SV40 was related to patients of malignant mesothelioma in Japan by the polymerase chain reaction (PCR) assay, DNA sequence analysis, and immunohistochemical methods. Paraffin-embedded samples of the 18 autopsied patients with pleural malignant mesothelioma were collected from five hospitals in Japan. After isolation of DNA from paraffin blocks, PCR analyses followed by sequencing were performed using three different sets of primers for detection of SV40 large T antigen (TAg) gene. All 18 malignant mesothelioma samples were also immunohistochemically evaluated for expression of SV40 TAg protein with two different anti-SV40 TAg antibodies. SV40 TAg genome was detected in eight malignant mesothelioma cases. Only one of three primer pairs successfully amplified SV40 genome in the samples, whereas all pairs yielded a PCR product in the controls, suggesting a low content of virus DNA. No immunopositive staining for SV40 TAg was found in any of the samples. This study shows that SV40 genome was present in a subset of Japanese malignant mesothelioma patients who were unlikely to have received a contaminated polio vaccine based on their age.