Remotely Activated DNA Probe System for the Detection and Imaging of Dual miRNAs.
Remotely Activated DNA Probe System for the Detection and Imaging of Dual miRNAs.
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DOI：
10.1021/acsabm.3c01079
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发表时间：
2023-12
影响因子：
4.7
通讯作者：
Yan Zhong;Zhihao Li;Zheng Li;Bo-lun Li;Hui Xin;Chun-yang Wang
中科院分区：
文献类型：
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作者：
Yan Zhong;Zhihao Li;Zheng Li;Bo-lun Li;Hui Xin;Chun-yang Wang
癌症仍然是全世界死亡的主要原因。在早期发现癌症对于提高生存率至关重要。生物标志物对癌症进展有精确的影响。在这里，我们建立了一个简单的DNA探针系统，可以被近红外光激活，以高特异性检测双miRNA。这种探针是建立在上转换纳米粒子的基础上的，它可以发射紫外光并激活吸附在外层的DNA探针。DNA探针系统通过操纵近红外（NIR）光进行远程控制，从而能够同时检测双miRNA。DNA纳米系统可以被癌细胞有效地内吞，并反映双miRNA的表达水平。总的来说，这项研究证明了一种有前途的远程控制DNA纳米平台，用于同时检测双miRNA，这对精确的癌症诊断和治疗具有巨大的潜力。
Cancers remain the leading cause of mortality worldwide. It is crucial to detect cancer at an early stage for improving survival rates. Biomarkers have precise implications for cancer progression. Here, we built a straightforward DNA probe system that could be activated by near-infrared light to detect dual miRNAs with a high specificity. This probe is built on the basis of upconversion nanoparticles, which could emit ultraviolet light and activate DNA probes adsorbed on the outer layer. The DNA probe system is remotely controlled through manipulation of the near-infrared (NIR) light, enabling simultaneous detection of dual miRNAs. The DNA nanosystem could be effectively endocytosed by cancer cells and reflect expression levels of dual miRNAs. Overall, this study demonstrates a promising remote-controlled DNA nanoplatform for the simultaneous detection of dual miRNAs, which has tremendous potential for precise cancer diagnostics and therapies.