STUDIES ON SOUND-INDUCED EPILEPSY IN MICE
STUDIES ON SOUND-INDUCED EPILEPSY IN MICE
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DOI：
10.1098/rspb.1984.0028
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
JOHNSON, MS
中科院分区：
文献类型：
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作者：
CHUNG, SH;JOHNSON, MS
小鼠癫痫易感菌株（DBA/2J）脑内吡哆醛-5′-磷酸和二价过渡金属离子（Cu2+和Zn2+）浓度明显高于正常菌株（CBA/Ca和Parkes）。通过脑内注射金属离子和腹腔注射吡哆醛-5′-磷酸，使正常小鼠容易发生声致癫痫。接受治疗的老鼠对响亮声音的反应与易癫痫发作的老鼠本质上是相同的。下丘谷氨酸和天冬氨酸水平升高，γ-氨基丁酸盐浓度降低。谷氨酰胺酶抑制剂6-重氮-5-氧-低去甲亮氨酸（DON）和重氮-乙酰- l-丝氨酸（azaserine）以及转氨酶抑制剂4-氨基-3-异恶唑酮（l-环丝氨酸）腹腔注射可保护癫痫易感小鼠免受惊厥，而吡哆醛-5'-磷酸和甲硫氨酸亚砜（谷氨酰胺合成酶抑制剂）则加重其癫痫病情。我们提出了与听原性癫痫易感性相关的生化事件的可能序列。
The cerebral concentrations of pyridoxal-5'-phosphate and divalent transition metal ions (Cu2+and Zn2+) are appreciably higher in the seizure-susceptible strain of mouse (DBA/2J) than those in normal strains (CBA/Ca and Parkes). By injecting metal ions intracranially and pyridoxal-5'-phosphate intraperitoneally, we could render the normal mouse prone to sound-induced epilepsy. The behaviour of the treated mouse in response to a loud sound was essentially identical to that of the seizure-susceptible strain of mouse. The levels of glutamate and aspartate in its inferior colliculus were elevated and the concentration ofγ-aminobutyrate was lowered. Glutaminase inhibitors, 6-diazo-5-oxo-Lnorleucine (DON) ando-diazo-acetyl-L-serine (azaserine), and a transaminase inhibitor, 4-amino-3-isoxazolidone (L-cycloserine), when injected intraperitoneally, protected the seizure-susceptible mouse from undergoing convulsions, whereas pyridoxal-5'-phosphate and methionine sulphoximine, a glutamine synthetase inhibitor, exacerbated its epileptic condition. We propose a possible sequence of biochemical events associated with susceptibility to audiogenic seizures.