Characterization of tolloid-related-1: a BMP-1-like product that is required during larval and pupal stages of Drosophila development.
Characterization of tolloid-related-1: a BMP-1-like product that is required during larval and pupal stages of Drosophila development.
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tolloid-relative-1 的表征：一种 BMP-1 样产品，在果蝇发育的幼虫和蛹阶段是必需的。
DOI：
10.1006/dbio.1994.1338
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发表时间：
1994
影响因子：
2.7
通讯作者：
O'Connor,MB
中科院分区：
文献类型：
--
作者：
Nguyen,T;Jamal,J;Shimell,MJ;Arora,K;O'Connor,MB
The Drosophilatolloid (tld)gene product belongs to a family of developmentally important proteins that includes bone morphogenetic protein-1 (BMP-1). In Drosophila,tldis required at the blastoderm stage to establish pattern within the dorsal half of the embryo. Genetic analysis suggests that the major function oftldis to augment the activity of thedecapentaplegicgene product, a close relative of the TGF-β superfamily members, BMP-2 and BMP-4. In this report, we describe a new gene calledtolloid-related-1 (tlr-1)that maps immediately proximal totld. Sequence analysis indicates thattlr-1has a large N-terminal extension relative totld, but otherwise shows the same general organization of sequence motifs found intldand other BMP-1 family members. These include a region of similarity to astacin, a crayfish metalloprotease, five copies of a repeat first found in complement proteins C1r and C1s, and two copies of an epidermal growth factor-like sequence.In situhybridization experiments show thattlr-1expression partially overlapstldexpression in early embryos, but shows unique transcriptional patterns in late stage embryos that are not seen withtld. In larval stages, both genes are expressed in identical patterns in imaginal discs and in the optic lobes of the brain, buttlr-1is more abundant thantld. Deletions that eliminatetlr-1expression cause lethality during larval and pupal stages of development. A small proportion of homozygous mutant flies eclose and show wing veination defects. Transgenic animals in which atlr-1cDNA is driven by thetldpromoter fail to rescue tld mutations, and extra copies of tld fail to rescuetlr-1mutations, implying that these genes have evolved functionally distinct features. We propose thattldandtlr-1arose by gene duplication and that each has evolved independently to acquire distinct tissue specific roles in Drosophila development.
