Human parechovirus infections in patients admitted to hospital in Northern Italy, 2008-2010
Human parechovirus infections in patients admitted to hospital in Northern Italy, 2008-2010
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DOI：
10.1002/jmv.23197
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发表时间：
2012-04-01
影响因子：
12.7
通讯作者：
Baldanti, Fausto
中科院分区：
文献类型：
--
作者：
Piralla, Antonio;Furione, Milena;Baldanti, Fausto
人类副病毒(HPeV)感染与一系列临床症状有关，如呼吸道、胃肠道、神经系统疾病和新生儿败血症样疾病。本研究的主要目的是调查2年间住院患者HPeV感染的流行病学情况。对2008年10月至2010年10月收集的3525例呼吸综合征患者的呼吸道样本、340例神经综合征患者的脑脊液(CSF)以及5例新生儿败血症样疾病患者的脑脊液(CSF)和血浆样本进行了HPeV特异性实时RT-PCR回顾性检测。对阳性标本进行VP3/VP1区系统发育分析。在3,525例呼吸综合征患者中有14例(0.4%)和5例败血症样疾病患者中有5例HPeV阳性。有3/5的败血症患者可获得多种样本(如粪便、血浆、脑脊液或呼吸道样本)，所有样本均检出HPeV。340例神经综合征患者脑脊液均未检出阳性。HPeV1株感染11例(57.9%)，HPeV3株感染7例(36.8%)，HPeV6株感染1例(5.3%)。在14名患有呼吸综合征的HPeV患者中，有10人同时感染了其他呼吸道病毒(8人感染鼻病毒，2人感染冠状病毒OC43)。所有5名脓毒症样疾病的患者年龄都不到1个月，都感染了HPeV3。尽管HPeV的传播频率不高，也不太可能导致呼吸综合征，但在少数新生儿中，HPeV与严重的临床症状有关。J.Med.维罗尔。84：686690,2012年。(C)2011年威利期刊公司。
Human parechoviruses (HPeVs) infection is associated with a wide range of clinical syndromes such as respiratory, gastrointestinal, neurologic diseases, and neonatal sepsis-like illness. The main objective of this study was to investigate the epidemiology of HPeVs infection in hospitalized patients in a period of 2 years. Respiratory samples from 3,525 patients with respiratory syndrome, cerebrospinal fluid (CSF) from 340 patients with neurologic syndrome as well as CSF and plasma samples from five neonatal patients with sepsis-like illness collected from October 2008 to 2010 were tested retrospectively using HPeV-specific real-time RT-PCR. Phylogenetic analysis of VP3/VP1 region was performed on the positive samples. Fourteen out of 3,525 (0.4%) patients with respiratory syndrome and five out of five patients with sepsis-like illness were positive for HPeV. In 3/5 patients with sepsis-like illness multiple samples (e.g., stool, plasma, CSF, or respiratory samples) were available, and HPeV was found in all specimens. In contrast, no positive CSF was detected among the 340 patients with neurologic syndromes. Eleven patients (57.9%) were infected with HPeV1 strain, 7 (36.8%) with HPeV3, and 1 (5.3%) with HPeV6 strains. Ten of the 14 HPeV patients with respiratory syndrome were co-infected with other respiratory viruses (eight with rhinovirus and two with coronavirus OC43). All five patients with sepsis-like illness were less than 1 month of age and were infected with HPeV3. Although not circulating at high frequency and unlikely to cause respiratory syndrome, HPeV was associated with severe clinical syndromes in a minority of newborns. J. Med. Virol. 84:686690, 2012. (C) 2011 Wiley Periodicals, Inc.