Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen
Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen
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DOI：
10.1104/pp.104.047019
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发表时间：
2004-09-01
期刊：
影响因子：
7.4
通讯作者：
Stitt, M
中科院分区：
文献类型：
--
作者：
Scheible, WR;Morcuende, R;Stitt, M
利用Affymetrix ATH1阵列和实时逆转录pcr平台对> 1400个转录因子进行转录组分析，以确定长期氮剥夺或短期硝酸盐营养对拟南芥的影响过程。两天的氮剥夺导致大部分参与光合作用、叶绿素合成、质体蛋白质合成的基因被协同抑制，许多参与二次代谢的基因被诱导，线粒体电子传递被重编程。硝酸盐的添加导致了快速、广泛和协调的变化。在主要代谢物发生显著变化之前，在30分钟内诱导了硝酸盐的吸收和还原、还原当量的产生和有机酸骨架的多个基因。到3 h时，大部分氨基酸和核苷酸的生物合成和清除基因被诱导，而大部分氨基酸和核苷酸的生物分解基因被抑制。许多参与RNA合成和加工的基因和大部分参与氨基酸激活和蛋白质合成的基因被协调诱导。虽然参与中枢代谢的氨基酸增加，但次要氨基酸减少，为蛋白质合成的激活提供了独立的证据。特异基因编码扩张蛋白和张力质体内在蛋白，表明硝酸盐营养激活了细胞的扩张和生长。许多可能参与调控的基因，包括海藻糖代谢和激素代谢基因、蛋白激酶和磷酸酶、受体激酶和转录因子的表达都有快速反应。
Transcriptome analysis, using Affymetrix ATH1 arrays and a real-time reverse transcription-PCR platform for >1,400 transcription factors, was performed to identify processes affected by long-term nitrogen-deprivation or short-term nitrate nutrition in Arabidopsis. Two days of nitrogen deprivation led to coordinate repression of the majority of the genes assigned to photosynthesis, chlorophyll synthesis, plastid protein synthesis, induction of many genes for secondary metabolism, and reprogramming of mitochondrial electron transport. Nitrate readdition led to rapid, widespread, and coordinated changes. Multiple genes for the uptake and reduction of nitrate, the generation of reducing equivalents, and organic acid skeletons were induced within 30 min, before primary metabolites changed significantly. By 3 h, most genes assigned to amino acid and nucleotide biosynthesis and scavenging were induced, while most genes assigned to amino acid and nucleotide breakdown were repressed. There was coordinate induction of many genes assigned to RNA synthesis and processing and most of the genes assigned to amino acid activation and protein synthesis. Although amino acids involved in central metabolism increased, minor amino acids decreased, providing independent evidence for the activation of protein synthesis. Specific genes encoding expansin and tonoplast intrinsic proteins were induced, indicating activation of cell expansion and growth in response to nitrate nutrition. There were rapid responses in the expression of many genes potentially involved in regulation, including genes for trehalose metabolism and hormone metabolism, protein kinases and phosphatases, receptor kinases, and transcription factors.