High expression of ganglioside alpha-2,8-sialyltransferase (GD3 synthase) gene in adult T-cell leukemia cells unrelated to the gene expression of human T-lymphotropic virus type I.
High expression of ganglioside alpha-2,8-sialyltransferase (GD3 synthase) gene in adult T-cell leukemia cells unrelated to the gene expression of human T-lymphotropic virus type I.
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成人 T 细胞白血病细胞中神经节苷脂 α-2,8-唾液酸转移酶（GD3 合酶）基因的高表达与人类 T 淋巴细胞病毒 I 型的基因表达无关。
作者：
M. Okada;K. Furukawa;S. Yamashiro;Y. Yamada;M. Haraguchi;K. Horibe;K. Kato;Y. Tsuji
分析了人白血病细胞中神经节苷脂α-2，8-唾液酸转移酶（GD 3合成酶; EC 2.4.99.8）基因和神经节苷脂的表达。使用单克隆抗体的各种类型的白血病细胞的流式细胞仪分析显示，GD 3在成人T细胞白血病（ATL）细胞上以中等水平表达，在T细胞急性淋巴细胞白血病细胞上表达较弱。因此，半定量逆转录-PCR分析表明ATL中GD 3合酶mRNA水平高，T细胞急性淋巴细胞白血病中GD 3合酶mRNA水平低。在未培养状态下，ATL细胞中GD 3合酶基因的高水平表达被检测到，并且在体外短期培养期间没有改变。与此相反，在培养的正常T淋巴细胞中，在有或无HTLV-I p40 tax蛋白的情况下，在人类T淋巴细胞病毒I型（HTLV-I）基因表达出现后，在培养开始后24小时内观察到GM 2/GD 2合酶基因的快速上调，GD 3合酶基因几乎相等地表达，而GM 2/GD 2合酶基因在p40 tax阳性T细胞中表达更强。这些发现表明GD 3合酶基因在ATL细胞中高度表达，与HTLV-I基因组表达无关。
Expression of ganglioside alpha-2,8-sialyltransferase (GD3 synthetase; EC 2.4.99.8) gene and gangliosides in human leukemia cells were analyzed. Flow cytometric analysis of various types of leukemia cells using monoclonal antibodies revealed that GD3 was expressed at a moderate level on adult T-cell leukemia (ATL) cells and weakly on T-cell acute lymphocytic leukemia cells. Accordingly, high levels of GD3 synthase mRNA in ATL and low levels in T-cell acute lymphocytic leukemia were demonstrated by semiquantitative reverse transcription-PCR analysis. High-level expression of the GD3 synthase gene in ATL cells was detected in the uncultured state, and was not changed during short-term culture in vitro. This was in contrast with the rapid up-regulation of GM2/GD2 synthase gene, which was observed within 24 h after start of culture after the emergence of human T-lymphotropic virus type I (HTLV-I) gene expression in cultured normal T lymphocytes with or without HTLV-I p40tax protein, GD3 synthase gene was almost equally expressed, whereas GM2/GD2 synthase gene was much more strongly expressed in the p40tax-positive T cells. These findings indicated that the GD3 synthase gene was highly expressed in ATL cells unrelated to HTLV-I genome expression.