In situ fluorescence microscopy visualization and characterization of nanometer-scale carbon nanotubes labeled with 1-pyrenebutanoic acid, succinimidyl ester
In situ fluorescence microscopy visualization and characterization of nanometer-scale carbon nanotubes labeled with 1-pyrenebutanoic acid, succinimidyl ester
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DOI：
10.1063/1.2206875
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发表时间：
2006-05-22
影响因子：
4
通讯作者：
Deaton, Russell
中科院分区：
文献类型：
--
作者：
Kim, Jin-Woo;Kotagiri, Nalinikanth;Deaton, Russell
为了表征利用碳纳米管(CNTs)的生物/无机复合技术，需要原位、实时、非侵入性的准确和可靠的成像方法来观察碳纳米管与生物材料(即DNA)在生物相关的水环境中的相互作用。本研究利用丁二酸丁二醇酯(PSE)和常规荧光显微镜对单个碳纳米管在水溶液中的光学可视化和表征进行了探索。结果表明，基于PSE染色方法的荧光显微镜具有监测单个碳纳米管的纳米分辨率的潜力，以及在生物/生物杂化系统中对生物材料的操纵。(C)2006年美国物理研究所。
In order to characterize hybrid bio/abio technology utilizing carbon nanotubes (CNTs), in situ, real-time, yet noninvasive methods of accurate and reliable imaging are needed for observing CNTs' interactions with biological materials, i.e., DNA, in biologically relevant aqueous environments. Optical visualization and characterization of individual CNTs in aqueous solutions were explored in this study using 1-pyrenebutanoic acid, succinimidyl ester (PSE) and a conventional fluorescence microscope. The results demonstrate the potential of fluorescence microscopy based on PSE-based staining methodology monitoring with nanometer resolution of individual CNTs and their manipulation with biological materials in bio/abio hybrid systems. (c) 2006 American Institute of Physics.