Comparison of the microbial community structure between inflamed and non-inflamed sites in patients with ulcerative colitis
Comparison of the microbial community structure between inflamed and non-inflamed sites in patients with ulcerative colitis
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DOI：
10.1111/jgh.14129
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发表时间：
2018-09-01
影响因子：
4.1
通讯作者：
Esaki, Motohiro
中科院分区：
文献类型：
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作者：
Hirano, Atsushi;Umeno, Junji;Esaki, Motohiro
背景与目的肠道菌群在溃疡性结肠炎（UC）的发病机制中起重要作用。然而，个体间和空间差异阻碍了uc相关变化的识别。因此，我们研究了从UC患者的炎症和非炎症部位以及非炎症性肠病（IBD）对照的相应部位获得的成对粘膜相关微生物群。方法对14例左侧或直肠炎型活动性UC患者进行了炎症部位和非炎症部位的粘膜活检。对14名非ibd对照者进行相应部位的配对粘膜活检。利用16S核糖体RNA基因序列分析微生物群落结构，并利用qiime和LEfSe软件进行数据分析。结果UC患者炎症部位和非炎症部位的微生物α多样性均显著低于非ibd对照组。UC患者炎症部位与非炎症部位相比，Cloacibacterium属和Tissierellaceae科微生物较多，而Neisseria属微生物较少。与非ibd对照组的相应部位相比，UC患者炎症部位的普雷沃菌属、真杆菌属、奈瑟菌属、细毛菌属、Bilophila属、Desulfovibrio属和Butyricimonas属的丰度明显降低。在这些分类群中，与非ibd对照组的相应部位相比，UC患者非炎症部位的Prevotella属和Butyricimonas属的数量也较少。结论UC患者在炎症部位和非炎症部位均发生粘膜微生物生态失调。UC患者中显示丰度改变的类群可能介导结肠炎症。
Background and AimThe gut microbiota is suggested to play an important role in the pathogenesis of ulcerative colitis (UC). However, interindividual and spatial variations hamper the identification of UC-related changes. We thus investigated paired mucosa-associated microbiota obtained from both inflamed and non-inflamed sites of UC patients and corresponding sites of non-inflammatory bowel disease (IBD) controls.MethodsMucosal biopsies of both inflamed and non-inflamed sites were obtained from 14 patients with active UC of the left-sided or proctitis type. Paired mucosal biopsies of the corresponding sites were obtained from 14 non-IBD controls. The microbial community structure was investigated using 16S ribosomal RNA gene sequences, followed by data analysis using qiime and LEfSe softwares.ResultsMicrobial alpha diversity in both inflamed and non-inflamed sites was significantly lower in UC patients compared with non-IBD controls. There were more microbes of the genus Cloacibacterium and the Tissierellaceae family, and there were less microbes of the genus Neisseria at the inflamed site when compared with the non-inflamed site in UC patients. Decreased abundance of the genera Prevotella, Eubacterium, Neisseria, Leptotrichia, Bilophila, Desulfovibrio, and Butyricimonas was evident at the inflamed site of UC patients compared with the corresponding site of non-IBD controls. Among these taxa, the genera Prevotella and Butyricimonas were also less abundant at the non-inflamed site of UC patients compared with the corresponding site in non-IBD controls.ConclusionsMucosal microbial dysbiosis occurs at both inflamed and non-inflamed sites in UC patients. The taxa showing altered abundance in UC patients might mediate colonic inflammation.