Preparation of continuous beds derivatized with one-step alkyl and sulfonate groups for capillary electrochromatography
Preparation of continuous beds derivatized with one-step alkyl and sulfonate groups for capillary electrochromatography
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DOI：
10.1021/ac960261k
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发表时间：
1996-10-01
影响因子：
7.4
通讯作者：
Hjerten, S
中科院分区：
文献类型：
--
作者：
Liao, JL;Chen, N;Hjerten, S
本文介绍了一种制备毛细管电色谱柱的简单一步法。通过添加表面活性剂使用于引入疏水配体（C18，C4）的甲基丙烯酸硬脂基酯或甲基丙烯酸丁酯在用于合成基质的含水单体混合物中水溶，将该溶液吸入一段熔融石英管中，其内壁被甲基丙烯酰基活化。在聚合之后，连续床柱准备好使用。由于床共价连接到管壁，因此不需要撞击来支撑床，这简化了柱的制备。此外，玻璃料可能容易堵塞，并且通常是产生气泡的场所。本文提出了一些提高电色谱分离度和缩短分析时间的新方法，如锐化起始区、采用梯度洗脱或在移动的相中加入SDS等。柱
A simple one-step procedure for the preparation of columns for capillary electrochromatography is described, The nonpolar compounds (stearyl or butyl methacrylate) used for the introduction of hydrophobic ligands (C18, C4) are rendered water-soluble in the aqueous monomer mixture used for the synthesis of the matrix by the addition of a surfactant, This solution is sucked into a piece of fused silica tubing, the inner walls of which are activated by methacryl groups. Following polymerization, the continuous bed column is ready for use, Since the bed is attached covalently to the tubing wall, no hit to support the bed is required, which simplifies the preparation of the column. In addition, a frit can easily become clogged and is often a site for the generation of air bubbles. Some new approaches to increase the resolution and shorten the analysis times in electrochromatography are suggested and demonstrated experimentally, such as sharpening the starting zone, employing gradient elution, or adding SDS (below cmc) to the mobile phase, Separation of five polycyclic aromatic hydrocarbons can be achieved in 5 min in a 25-mu m-i.d. column.