maternally expressed gene1 is a novel maize endosperm transfer cell-specific gene with a maternal parent-of-origin pattern of expression
maternally expressed gene1 is a novel maize endosperm transfer cell-specific gene with a maternal parent-of-origin pattern of expression
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DOI：
10.1105/tpc.019778
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发表时间：
2004-05-01
期刊：
影响因子：
11.6
通讯作者：
Dickinson, HG
中科院分区：
文献类型：
--
作者：
Gutiérrez-Marcos, JF;Costa, LM;Dickinson, HG
玉米(Zea Mays)胚乳的生长受到母体合子和孢子体基因的严格调控，其中一些基因受到亲本效应的影响。我们在这里报道了一个新的基因，母系表达基因1(Meg1)，它在胚乳发育的早期表现为母本起源的表达模式，但在后期表现为双等位基因表达。有趣的是，包含me1启动子的稳定报告融合显示了类似的表达模式。Meg1仅在胚乳的基转移区表达。此外，我们证明了假定加工的MEG1蛋白是糖基化的，随后定位于转移细胞壁的迷宫内生。因此，一个只在基本转移区表达的亲本起源基因的发现，打开了在胚乳中运行的表观遗传机制的大门，以调节从母体组织到发育中的种子的营养运输的某些方面。
Growth of the maize (Zea mays) endosperm is tightly regulated by maternal zygotic and sporophytic genes, some of which are subject to a parent-of-origin effect. We report here a novel gene, maternally expressed gene1 (meg1), which shows a maternal parent-of-origin expression pattern during early stages of endosperm development but biallelic expression at later stages. Interestingly, a stable reporter fusion containing the meg1 promoter exhibits a similar pattern of expression. meg1 is exclusively expressed in the basal transfer region of the endosperm. Further, we show that the putatively processed MEG1 protein is glycosylated and subsequently localized to the labyrinthine ingrowths of the transfer cell walls. Hence, the discovery of a parent-of-origin gene expressed solely in the basal transfer region opens the door to epigenetic mechanisms operating in the endosperm to regulate certain aspects of nutrient trafficking from the maternal tissue into the developing seed.