Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats
Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats
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布鲁氏菌血清型 A、M 与小肠结肠炎耶尔森氏菌 O:9 多糖对牛、绵羊、山羊布鲁氏菌病血清学诊断的比较分析
DOI：
10.1128/jcm.31.12.3136-3141.1993
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发表时间：
1993
影响因子：
9.4
通讯作者：
I. Moriyón
中科院分区：
文献类型：
--
作者：
E. Díaz;V. Aragon;C. Marín;B. Alonso;M. Font;E. Moreno;S. Pérez;J. Blasco;R. Díaz;I. Moriyón
从布鲁氏菌属中制备了布鲁氏菌平滑 M 和 A 血清型的半抗原多糖。和小肠结肠炎耶尔森氏菌 O:9，通过先前描述的水解（O 链）或非水解（天然半抗原 [NH]）程序。纯化的多糖仅在脂多糖核心糖的存在（O链）或不存在（NH）方面有所不同。通过反向径向免疫扩散比较多糖，以诊断牛（流产布鲁氏菌生物型 1 [A 血清型] 和羊布鲁氏菌生物型 3 [AM 血清型]）、绵羊（羊布鲁氏菌生物型 1 [M 血清型] 和 3）和山羊（羊布鲁氏菌生物型 1）。无论宿主物种和感染布鲁氏菌属的血清型如何，使用来自羊种布鲁氏菌 16 M（血清型 M）的 NH 进行的反向径向免疫扩散试验显示出最高的灵敏度（89.6% 至 97.3%）。小肠结肠炎耶尔森氏菌 O:9 NH（A 血清型）可用于诊断感染流产布鲁氏菌生物型 1 的牛的疾病，但不适用于诊断感染羊种布鲁氏菌生物型 3 的牛、绵羊或山羊。使用 M 和 A 血清型多糖以及感染布鲁氏菌属 M、A 和 AM 血清型的动物血清获得了不同的结果。结果表明，在自然感染的动物中，大部分抗体针对先前定义的与 A 或 M 表位不同的共同表位（J. T. Douglas 和 D. A. Palmer，J. Clin. Microbiol. 26：1353-1356， 1988）或与之反应。通过使用羊种布鲁氏菌 16M NH 进行放射状免疫扩散试验，可以区分感染者和接种疫苗的牛、绵羊和山羊，其敏感性和特异性与补体结合试验相似。
Hapten polysaccharides of Brucella smooth M and A serotypes were prepared from Brucella sp. and Yersinia enterocolitica O:9 by previously described hydrolytic (O chain) or nonhydrolytic (native hapten [NH]) procedures. The purified polysaccharides differed only in the presence (O chain) or absence (NH) of lipopolysaccharide core sugars. The polysaccharides were compared by reverse radial immunodiffusion for the diagnosis of brucellosis in cattle (Brucella abortus biotype 1 [A serotype] and Brucella melitensis biotype 3 [AM serotype]), sheep (B. melitensis biotypes 1 [M serotype] and 3), and goats (B. melitensis biotype 1). The reverse radial immunodiffusion test with the NH from B. melitensis 16 M (serotype M) showed the highest sensitivity (89.6 to 97.3%), regardless of the host species and the serotype of the infecting Brucella sp. Y. enterocolitica O:9 NH (A serotype) was useful for diagnosing disease in cattle infected with B. abortus biotype 1, but not in cattle infected with B. melitensis biotype 3, sheep, or goats. The different results obtained with the serotype M and A polysaccharides and the sera from animals infected with M, A, and AM serotypes of Brucella spp. showed that in naturally infected animals, a large proportion of the antibodies are directed to or react with a previously defined common epitope(s) (J. T. Douglas and D. A. Palmer, J. Clin. Microbiol. 26:1353-1356, 1988) different from the A or M epitopes. By using the radial immunodiffusion test with B. melitensis 16M NH, it was possible to differentiate infected from vaccinated cattle, sheep, and goats with a sensitivity and specificity similar to that of the complement fixation test.