Scanning Electron Microscopy of Escherichia coli Encapsulated in a Spacerized Graphene Sandwich
Scanning Electron Microscopy of Escherichia coli Encapsulated in a Spacerized Graphene Sandwich
复制标题
扫描电子显微镜观察封装在间隔石墨烯三明治中的大肠杆菌
DOI：
10.1093/jmicro/dfac010
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发表时间：
2022
期刊：
影响因子：
1.8
通讯作者：
Ryoma
中科院分区：
文献类型：
--
作者：
Sasaki;Yuki; Hirayama;Satoru; Nakao;Ryoma
细菌等生物材料的电子显微镜可以对其进行多方面的分析，以高分辨率了解其结构和功能，这是光学显微镜难以实现的。然而，在电子束辐照和真空环境下，样品会被破坏或破碎。在这里，我们观察到包裹在石墨烯三明治中的悬浮液中的细菌，该三明治可以在不需要固定的情况下防止电子束损伤。具体来说，我们用扫描电镜观察了大肠杆菌在石墨烯夹心层中的存在，该夹心层含有穿孔膜作为间隔层，封装了非固定化的ede。石墨烯层之间的碰撞。然而,E。虽然在最佳培养条件下重悬辐照后的细胞略有生长，但未观察到共作用，如分裂。我们的研究结果表明，石墨烯三明治方法可以观察wetE。电子显微镜下的结肠细胞，但需要改进，以允许生物材料的实时成像。
Electron microscopy of biological materials such as bacteria allows multifaceted analysis to understand their structure and function with high resolution, which is difficult to achieve with optical microscopy. However, the samples are damaged or broken by electron beam irradiation and by the vacuum environment. Here, we observed bacteria in a suspension encapsulated in a graphene sandwich that prevents electron beam damage without the need for fixation. Specifically, we demonstratedin situscanning electron microscopy observation ofEscherichia coliin a graphene sandwich containing a perforated membrane as a spacer, encapsulating non-immobilizedE. colibetween the graphene layers. However,E. coliactivity, such as division, was not observed, although the irradiated cells grew slightly when resuspended under optimal culture conditions. Our findings suggest that the graphene sandwich methodology enables the observation of wetE. colicells by electron microscopy but requires refinement to allow the live imaging of biological materials.