Development of a flavoprotein column for chiral separation by high-performance liquid chromatography
Development of a flavoprotein column for chiral separation by high-performance liquid chromatography
复制标题
高效液相色谱手性分离黄素蛋白柱的研制
DOI：
10.1016/0021-9673(92)80361-w
复制
发表时间：
1992
影响因子：
4.1
通讯作者：
T. Miwa
中科院分区：
文献类型：
--
作者：
N. Mano;Y. Oda;N. Asakawa;Y. Yoshida;Tadashi Sato*;T. Miwa
以鸡蛋清中的糖蛋白黄素蛋白为手性固定相，制备了一种新的高效液相色谱手性固定相。该柱可实现酸性（酮洛芬）、弱酸性（华法林）和中性（安息香）化合物的基线分离。在黄素蛋白偶联硅胶柱中，几种模型药物的容量因子和对映体选择性受移动的相中有机溶剂和盐的浓度、pH值的影响较大，不同化合物的最佳手性分离条件不同。然而，该柱显示出良好的稳定性，pH值的变化和有机溶剂，它应该是适用于许多化合物的手性分离在反相模式。该色谱柱可直接以水为移动的流动相分离光学异构体，在药物动力学和临床化学等领域具有重要的应用价值。
A new chiral stationary phase using flavoprotein, a glycoprotein present in chicken egg-white, was developed for high-performance liquid chromatography. This column could achieve baseline separations of acidic (ketoprofen), weakly acidic (warfarin) and neutral (benzoin) compounds. In the flavoprotein-conjugated silica gel column, the capacity factor and enantioselectivity for several model drugs were greatly influenced by the pH and the concentration of organic solvents and salts in the mobile phase, and the optimum conditions for chiral separation varied from compound. However, this column shows good stability to pH variation and to organic solvents, and it should be applicable for the chiral separation of many compounds in the reversed-phase mode. This column can directly separate optical isomers with an aqueous mobile phase, so it should be very useful in the fields of pharmacokinetics and clinical chemistry.