Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus.
Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus.
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爪蟾荧光标记免疫细胞的过继转移。
DOI：
10.1101/pdb.prot097592
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发表时间：
2019
影响因子：
--
通讯作者：
Robert,Jacques
中科院分区：
文献类型：
--
作者：
Rhoo,KunHyoe;Robert,Jacques
从近交系非洲爪蟾成体到近交系蝌蚪的免疫细胞转移是研究免疫细胞或病原体感染免疫细胞在蝌蚪体内传播的有效方法。例如，非洲爪蟾腹膜白细胞（PL）可以容易地被病原体如蛙病毒3（FV3）和海洋分枝杆菌（M. marinum）。通过将荧光标记的FV 3感染的PL转移到蝌蚪中，我们观察到这些细胞浸润到蝌蚪的脑中，这表明FV 3感染的PL可以穿过血脑屏障。利用蝌蚪的透明性，荧光标记的免疫细胞可以使用荧光显微镜进行真实的实时跟踪。
Adoptive cell transfer from inbred adult Xenopus to inbred tadpoles is a useful way to study the dissemination of immune cells or pathogen-infected immune cells in tadpoles. For example, Xenopus peritoneal leukocytes (PLs) can be readily infected by pathogens such as Frog virus 3 (FV3) and Mycobacterium marinum (M. marinum). By transferring fluorescently labeled, FV3-infected PLs into tadpoles, we observed infiltration of these cells into the tadpole's brain, which indicates that FV3-infected PLs can cross blood brain barrier. Taking advantage of tadpoles’ transparency, fluorescently labeled immune cells can be tracked in real time using fluorescence microscopy.