OBSERVATIONS ON THE ABSORPTION SPECTRA OF FLUORESCEIN, FLUORESCEIN DERIVATIVES AND CONJUGATES
OBSERVATIONS ON THE ABSORPTION SPECTRA OF FLUORESCEIN, FLUORESCEIN DERIVATIVES AND CONJUGATES
复制标题
DOI：
10.1016/0003-9861(58)90234-0
复制
发表时间：
1958-01-01
影响因子：
3.9
通讯作者：
EMMART, EW
中科院分区：
文献类型：
--
作者：
EMMART, EW
当pH值低于7时，荧光素在490 m[mu]处的特征吸收最大值迅速降低，在pH值为2时完全消失。在pH 7.6下，荧光素溶液在490 m[mu]处有最大的吸收带，在280和325 m[mu]处有较小的吸收带。通过与荧光素胺II制备的异氰酸荧光素结合，实现了球蛋白与荧光素核的成功结合。后者的特征在于其红外光谱，并与a . H. Coons教授（哈佛医学院）实验室的标准样品进行了比较。得到了荧光素胺和异氰酸荧光素在二氧环丙酮溶液中的紫外吸收光谱，并给出了荧光球蛋白偶联物的紫外吸收光谱。
With fall in pH below 7 the characteristic absorption maximum of fluorescein at 490 m[mu], is rapidly reduced and disappears completely at pH 2. At pH 7.6 solutions of fluorescein have an absorption maximum at 490 m[mu] with minor bands at 280 and 325 m[mu]. Successful conjugation of globulin to the fluorescein nucleus is accomplished by combination with fluorescein isocyanate which is prepared from fluorescein amine II. The latter has been characterized by its infra red spectrum and a comparison made with the standard sample from the laboratory of Prof. A. H. Coons (Harvard Med. School). The UV absorption spectrum of fluorescein amine II and fluorescein isocyanate in dioxane acetone solution has been obtained, and the absorption spectra of fluorescent globulin conjugates given.