Examination of the Catabolic Rates of 13C-Labeled Fatty Acids Bound to the α and β Positions of Triacylglycerol Using 13CO2 Expired from Mice.
Examination of the Catabolic Rates of 13C-Labeled Fatty Acids Bound to the α and β Positions of Triacylglycerol Using 13CO2 Expired from Mice.
复制标题
使用小鼠体内过期的 13CO2 检查与三酰甘油的 α 和 β 位结合的 13C 标记脂肪酸的分解代谢速率。
作者：
Kazuaki Yoshinaga;Fumiaki Beppu;Yoshio Yamatani;A. Kubo;Aya Yoshinaga;T. Nagai;Akihiko Yoshida;J. Kanda;N. Gotoh
三酰甘油(TAG)中的脂肪酸在消化后被分解。然而，结合在TAG的α(sn-1，3)或β(sn-2)位置上的几种脂肪酸的分解代谢率还没有得到彻底的比较。本研究用同位素比值质谱仪比较了~(13)C标记的棕榈酸、油酸、亚油酸、α-亚麻酸、二十碳五烯酸或二十二碳六烯酸与TAG的α和β结合的分解速率。用小鼠呼出的二氧化碳中13C和12C的比率来评估所研究的脂肪酸的分解速率。结果表明，棕榈酸、油酸或α-亚麻酸与β结合时相比与α结合时相比，在较长时间内降解缓慢。相比之下，与β立场结合的环保局很快被分解，而与α立场结合的环保局在很长一段时间内被缓慢分解。对于亚油酸或DHA，在TAG的结合位置之间没有检测到分解代谢率的差异。此外，与其他脂肪酸相比，EPA和DHA的分解更少。这些结果表明，脂肪酸的分解速率受其在标签中的结合位置的影响，并且这种影响因脂肪酸种类的不同而不同。
Fatty acids in triacylglycerol (TAG) are catabolized after digestion. However, the catabolic rates of several fatty acids bound to the α (sn-1, 3) or β (sn-2) position of TAG have not been thoroughly compared. In this study, the catabolic rates of 13C-labeled palmitic acid, oleic acid, linoleic acid, α-linolenic acid, eicosapentaenoic acid (EPA), or docosahexaenoic acid (DHA) bound to the α and β position of TAG were compared using isotope ratio mass spectrometry. The catabolic rates of the studied fatty acids were evaluated using the ratio of 13C and 12C in carbon dioxide expired from mice. The results indicated that palmitic acid, oleic acid, or α-linolenic acid bound to the β position was slowly catabolized for a long duration compared to that when bound to the α position. In contrast, EPA bound to the β position was quickly catabolized, and EPA bound to the α position was slowly catabolized for a long time. For linoleic acid or DHA, no difference in the catabolic rates was detected between the binding positions in TAG. Furthermore, EPA and DHA were less catabolized than the other fatty acids. These results indicate that the catabolic rates of fatty acids are influenced by their binding positions in TAG and that this influence on the catabolic rate differed depending on the fatty acid species.