Passive protection against Burkholderia pseudomallei infection in mice by monoclonal antibodies against capsular polysaccharide, lipopolysaccharide or proteins
Passive protection against Burkholderia pseudomallei infection in mice by monoclonal antibodies against capsular polysaccharide, lipopolysaccharide or proteins
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DOI：
10.1099/0022-1317-51-12-1055
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发表时间：
2002-12-01
影响因子：
3
通讯作者：
Oyston, PCF
中科院分区：
文献类型：
--
作者：
Jones, SM;Ellis, JF;Oyston, PCF
类鼻疽伯克霍尔德菌是类鼻疽病的病原体，在东南亚和澳大利亚北方流行，是人类疾病的重要原因。没有可用的疫苗，抗生素治疗与高复发率相关。产生了一组识别荚膜多糖、脂多糖或蛋白质的七种单克隆抗体（MAb），并评价了它们被动保护小鼠免受实验性类鼻疽的能力。单克隆抗体能够保护小鼠免受10（4）cfu/250 MLD的B强毒株的腹膜内攻击。当合并时，四种MAb具有单独的保护性。然而，在较高的B。在10（6）cfu的类鼻疽杆菌攻击水平下，没有一种单克隆抗体提供保护，只有抗外泌多糖单克隆抗体产生显著延迟的死亡时间。
Burkholderia pseudomallei, the aetiological agent of melioidosis, is endemic in south-east Asia and northern Australia, where it is an important cause of human disease. There is no vaccine available and antibiotic therapy is associated with high relapse rates. A panel of seven monoclonal antibodies (MAbs) that recognise capsular polysaccharide, lipopolysaccharide or proteins was produced and their ability to protect mice passively against experimental melioidosis was evaluated. The MAbs were capable of protecting mice against intra-peritoneal challenge with 10(4) cfu/250 MLD of a virulent strain of B. pseudomallei (NCTC 4845), when pooled, and four of the MAbs were individually protective. However, at a higher B. pseudomallei challenge level of 10(6) cfu none of the MAbs afforded protection and only the anti-exopolysaccharide MAbs produced a significantly delayed time to death.