Simple, rapid and sensitive assay method for simultaneous quantification of urinary nicotine and cotinine using gas chromatography-mass spectrometry
Simple, rapid and sensitive assay method for simultaneous quantification of urinary nicotine and cotinine using gas chromatography-mass spectrometry
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DOI：
10.1016/j.jchromb.2006.07.029
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发表时间：
2006-12-05
影响因子：
3
通讯作者：
Awang, Rahmat
中科院分区：
文献类型：
--
作者：
Man, Che Nin;Gam, Lay-Harn;Awang, Rahmat
尼古丁是香烟中一种主要的成瘾化合物。它的烟雾在人体内被迅速和广泛地代谢成几种代谢物。可替宁作为尼古丁的主要代谢物，通常被用作判断主动吸烟者和被动吸烟者的生物标志物。可替宁的半衰期(类似于20小时)比尼古丁(类似于2小时)更长。建立了一种简便、灵敏、快速、高通量的GC-MS同时测定被动吸烟者和主动吸烟者尿尼古丁和可替宁的方法。在样品制备方法中，首先对分析物和内标进行碱化，然后进行液-液萃取。完成后，将无水硫酸钠加入到溶剂混合物中以捕捉水分。将得到的透明提取物直接进样GC-MS，在选择离子监测(SIM)模式下工作。建立了尿基质中分析物浓度在0.997.5~5 0 0 0 ng/m L范围内的标准曲线，线性相关系数r(2)>0.0 5。尼古丁和可替宁的检出限均为0.20 ng/m L。烟碱和可替宁的平均回收率分别为93.0%和100.4%。尼古丁的批内和批间精密度在2.1-7.9%之间，可替宁在0.7-11.1%之间。批内和批间精密度尼古丁为3.3-9.5%，可替宁为3.4-9.8%。该方法可用于主动吸烟和环境烟草烟雾暴露的常规评价和监测。在吸烟者和不吸烟者之间的小规模比较研究中，该检测的适用性得到了证明。(C)2006爱思唯尔B.V.保留所有权利。
Nicotine is a major addictive compound in cigarette. Its smoke is rapidly and extensively metabolized to several metabolites in human. Cotinine as a major metabolite of nicotine is commonly used as a biomarker to determine active and passive smokers. Cotinine has a longer half-life (similar to 20 h) compared to nicotine (similar to 2 h). A simple, sensitive, rapid and high throughput GC-MS method was developed for simultaneous quantification of urinary nicotine and cotinine in passive and active smokers. In the sample preparation method, the analytes and internal standard were first basified and followed by liquid-liquid extraction. Upon completion, anhydrous sodium sulphate was added to the solvent mixture to trap moistures. The clear extract obtained was directly injected into GC-MS, operating under selective ion monitoring (SIM) mode. Calibration curves in the range of 0.5-5000 ng/mL of the analytes in urine matrix were established with linear correlation coefficients (r(2)) greater than 0.997. The limit of detection for both nicotine and cotinine were 0.20 ng/mL. The mean recoveries for nicotine and cotinine were 93.0 and 100.4%, respectively. The within- and between-assay accuracies were between 2.1 and 7.9% for nicotine and between 0.7 and 11.1% for cotinine. Within- and between-assay precisions of 3.3-9.5% for nicotine and 3.4-9.8% for cotinine were also achieved. The method can be used in routine assessment and monitoring of active smoking and exposure to environmental tobacco smoke. The applicability of the assay was demonstrated in a small-scale comparison study between smokers and non-smokers. (c) 2006 Elsevier B.V. All rights reserved.