bgs2+, a sporulation-specific glucan synthase homologue is required for proper ascospore wall maturation in fission yeast
bgs2+, a sporulation-specific glucan synthase homologue is required for proper ascospore wall maturation in fission yeast
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DOI：
10.1046/j.1365-2958.2000.02118.x
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发表时间：
2000-10-01
影响因子：
3.6
通讯作者：
Sánchez, Y
中科院分区：
文献类型：
--
作者：
Martín, V;Ribas, JC;Sánchez, Y
粟酒裂殖酵母的子囊孢子细胞壁的形成需要参与其组分（例如葡聚糖）的生物合成的酶的协同活性。我们克隆了bgs 2（+）基因。bgs 2（+）属于S.粟酒裂殖酵母，与酿酒酵母FKS 1和FKS 2基因同源。该基因的缺失或过表达不会导致营养生长过程中的任何明显缺陷，但纯合bgs 2 δ二倍体确实显示出孢子形成缺陷。虽然减数分裂正常进行，但子囊孢子不能成熟，其壁与野生型子囊孢子不同。此外，bgs 2 δ合子不能自发释放子囊孢子，并且子囊孢子不能萌发。我们表明，bgs 2（+）的表达仅限于孢子形成和bgs 2-绿色荧光蛋白（GFP）融合蛋白定位于子囊孢子包膜。与野生型二倍体相比，bgs 2缺失的产孢二倍体中的葡聚糖合酶活性降低，这一事实强调了bgs 2（+）基因和葡聚糖合成对于子囊孢子壁的正确形成和成熟的重要性。
The formation of the ascospore cell wall of Schizosaccharomyces pombe requires the co-ordinated activity of enzymes involved in the biosynthesis of its components, such as glucans. We have cloned the bgs2(+) gene. bgs2(+) belongs to the glucan synthase family of S. pombe and is homologous to the Saccharomyces cerevisiae FKS1 and FKS2 genes. Deletion or overexpression of this gene does not lead to any apparent defect during vegetative growth, but homozygous bgs2 Delta diploids do show a sporulation defect. Although meiosis takes place normally, ascospores are unable to mature, and their wall differs from that of wild-type ascospores. Moreover, bgs2 Delta zygotes were not able to release ascospores spontaneously, and the ascospores were unable to germinate. We show that expression of bgs2(+) is restricted to sporulation and that a bgs2-green fluorescent protein (GFP) fusion protein localizes to the ascospore envelope. The glucan synthase activity in sporulating diploids bearing a bgs2 deletion was diminished in comparison with that of the wild-type diploids, a fact that underscores the importance of the bgs2(+) gene and glucan synthesis for the proper formation and maturation of the ascospore wall.