Cell cycle-regulated gene expression in Arabidopsis
Cell cycle-regulated gene expression in Arabidopsis
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DOI：
10.1074/jbc.m207570200
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发表时间：
2002-11-01
影响因子：
4.8
通讯作者：
Murray, JAH
中科院分区：
文献类型：
--
作者：
Menges, M;Hennig, L;Murray, JAH
调节的基因表达是控制酵母和哺乳动物细胞周期进程的重要机制，与细胞分裂相关过程涉及的基因通常显示出取决于细胞周期位置的转录调控。由于缺乏拟南芥的可同步细胞悬浮液，植物中细胞周期过程的分析受到了阻碍，并且知道细胞周期调节的基因很少。使用最近描述的同步系统，我们使用Affymetrix基因阵列分析了拟南芥细胞的顺序样品中的RNA。我们确定了将近500个基因，这些基因在表达中表现出明显的波动，代表了任何植物中细胞周期调节的基因表达的首次基因组分析。除了先前鉴定为植物中细胞周期调节的基因数量有限之外，我们还发现了已知或怀疑与信号转导，转录调控和激素调节有关的基因调节的特定模式，包括细胞分裂素反应的关键基因。所鉴定的基因表示在其他生物体中受细胞周期调节的途径以及参与植物特异性过程的途径。细胞周期调节的基因的范围和数量显示了植物细胞周期与各种细胞控制和反应途径的紧密整合。
Regulated gene expression is an important mechanism for controlling cell cycle progression in yeast and mammals, and genes involved in cell division-related processes often show transcriptional regulation dependent on cell cycle position. Analysis of cell cycle processes in plants has been hampered by the lack of synchronizable cell suspensions for Arabidopsis, and few cell cycle-regulated genes are known. Using a recently described synchrony system, we have analyzed RNA from sequential samples of Arabidopsis cells progressing through the cell cycle using Affymetrix Gene-arrays. We identify nearly 500 genes that robustly display significant fluctuation in expression, representing the first genomic analysis of cell cycle-regulated gene expression in any plant. In addition to the limited number of genes previously identified as cell cycle-regulated in plants, we also find specific patterns of regulation for genes known or suspected to be involved in signal transduction, transcriptional regulation, and hormonal regulation, including key genes of cytokinin response. Genes identified represent pathways that are cell cycle-regulated in other organisms and those involved in plant-specific processes. The range and number of cell cycle-regulated genes show the close integration of the plant cell cycle into a variety of cellular control and response pathways.