Plasmodium falciparum: induction of biologically active antibodies to gp195 is dependent on the choice of adjuvants.
Plasmodium falciparum: induction of biologically active antibodies to gp195 is dependent on the choice of adjuvants.
复制标题
恶性疟原虫：针对 gp195 的生物活性抗体的诱导取决于佐剂的选择。
DOI：
10.1016/0014-4894(92)90131-s
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发表时间：
1992
影响因子：
2.1
通讯作者：
Chang,SP
中科院分区：
文献类型：
--
作者：
Hui,GS;Chang,SP
研究了以合成免疫调节剂B30-MDP和LA-15-PH为基础的佐剂配方是否能影响小鼠产生具有生物活性的抗gp195(裂殖子表面主要蛋白OFP)。恶性疟原虫)抗体。用纯化的gp195以B30-MDP和LA-15-PH为佐剂，每隔21天免疫C57BL/10SNJ小鼠4次，获得小鼠血清。以明矾和CFA为参比佐剂。免疫前采集免疫前血清，第四次免疫后第14天和第21天采集免疫血清。在体外试验中，用不同浓度的小鼠血清添加15%的正常人血清不支持寄生虫的生长。为此，采用硫酸铵沉淀法从小鼠血清中分离纯化了总多克隆抗体，并进行了寄生虫生长抑制试验。正常的小鼠免疫球蛋白部分支持OFP的体外发育。恶性寄生虫。在LA-15-PH中用gp195免疫的小鼠的抗体在体外抑制了80%的寄生虫，而来自其他佐剂组的抗体几乎没有抑制作用(0-25%)。这一发现与以前在兔身上进行的研究结果形成了鲜明对比，LA-15-PH诱导了抑制性抗体，而其他佐剂没有。据认为，这一结果表明佐剂制剂对免疫反应质量的总体影响取决于物种。这些研究还强调了佐剂在免疫反应的特异性上的作用，以及更重要的是，在体内诱导可能表明保护性免疫的生物相关抗体群体的重要性。
It was investigated whether the adjuvant formulation based on the synthetic immunomodulators B30-MDP and LA-15-PH, can influence the induction of biologically active mouse anti-gp195 (the major merozoite surface protein ofP. falciparum) antibodies. Mouse sera were obtained from a previous study in which C57Bl/10 SnJ mice were immunized ip 4 times at 21-day intervals with purified gp195 in different adjuvant formulations based on B30-MDP and LA-15-PH. Alum and CFA were used as reference adjuvants. Preimmune sera were obtained prior to immunization and immune sera were collected 14 and 21 days after the quarternary immunization.In vitroassays using various concentrations of mouse sera supplemented with 15% normal human serum did not support parasite growth. Therefore total polyclonal antibodies from mouse sera were purified by ammonium sulfate precipitation and parasite growth inhibition assays were performed. Normal mouse immunoglobulin fractions supported thein vitrodevelopment ofP. falciparumparasites. Antibodies from mice immunized with gp195 in LA-15-PH inhibited >80% of the parasitesin vitrobut antibodies from other adjuvant groups produced little or no inhibition (0-25%). This finding that LA-15-PH induced inhibitory antibodies, while other adjuvants did not, contrasts with findings in previous studies with rabbits. It is believed that this result shows that the overall effect of adjuvant formulations on the quality of immune responses is species dependent. It is also concluded that these studies emphasize the significance of the role of adjuvants on the specificity of the immune response and, more importantly, on the induction of biologically relevant antibody populations which may be indicative of protective immunityin vivo.
佐剂对恶性疟原虫主要裂殖子表面蛋白 gp195 的抗体特异性的影响。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hui,GS;Chang,SP;Gibson,H;Hashimoto,A;Hashiro,C;Barr,PJ;Kotani,S
通讯作者：
Kotani,S
影响因子：
3.1
作者：
HUI, GSN;TAM, LQ;KOTANI, S
通讯作者：
KOTANI, S