An Evaluation of Methicillin-Resistant Staphylococcus aureus Survival on Five Environmental Surfaces
An Evaluation of Methicillin-Resistant Staphylococcus aureus Survival on Five Environmental Surfaces
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DOI：
10.1089/mdr.2011.0007
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发表时间：
2011-09-01
影响因子：
2.6
通讯作者：
Stetzenbach, Linda D.
中科院分区：
文献类型：
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作者：
Coughenour, Courtney;Stevens, Vanessa;Stetzenbach, Linda D.
这项研究评估了耐甲氧西林金黄色葡萄球菌(MRSA)在环境表面：玻璃、木材、乙烯基、塑料和布料上的生存情况。考察了相对湿度(RH)和牛血清白蛋白(BSA)的影响。表面接种10(7)-10(8)个/毫升的MRSA菌落形成单位(CFU/ml)，并在35℃、45%-55%和16%RH的条件下培养。对表面进行采样，将收集的每个样品重新悬浮在磷酸盐缓冲液中，涂抹电镀，并在35℃下孵化24小时；对产生的菌落进行计数。在干燥后立即、3小时、24小时、2天、3天、4天和5天采集样品。结果表明，耐甲氧西林金黄色葡萄球菌在塑料和乙烯基上存活时间最长，在木材上存活时间最短(p<；0.001)。牛血清白蛋白使耐甲氧西林金黄色葡萄球菌得以存活更长时间(p<；0.001)。45%-55%RH保存的表面CFU/ml明显低于16%RH保存的表面。这项研究表明，活的MRSA细菌可以在表面停留数天，这可能会影响工作场所和住宅环境中居住者的公共健康。
This study evaluated methicillin-resistant Staphylococcus aureus (MRSA) survival on environmental surfaces: glass, wood, vinyl, plastic, and cloth. Effects of relative humidity (RH) and bovine serum albumin (BSA) were examined. Surfaces were inoculated with 10(7)-10(8) colony forming units per milliliter (CFU/ml) of MRSA with and without 1% BSA and incubated at 35 degrees C at 45%-55% and 16% RH. Surfaces were sampled, and each collected sample was re-suspended in phosphate buffer, spread plated, and incubated at 35 degrees C for 24 hrs; resulting colonies were enumerated. Samples were collected immediately on drying, and at 3 hrs, 24 hrs, 2 days, 3 days, 4 days, and 5 days. Results demonstrated that MRSA survived the longest on plastic and vinyl and for the least amount of time on wood (p < 0.001). BSA enabled MRSA to survive for significantly longer duration (p < 0.001). The number of CFU/ml was significantly lesser on surfaces stored in 45%-55% RH versus 16% RH. This study demonstrates that viable MRSA bacteria can remain on surfaces for days, which may impact the public health of occupants in workplace and residential settings.