Kinetic analysis of carbon-11-labeled carbon dioxide for studying photosynthesis in a leaf using positron emitting tracer imaging system

Kinetic analysis of carbon-11-labeled carbon dioxide for studying photosynthesis in a leaf using positron emitting tracer imaging system
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DOI:
10.1109/tns.2006.881063
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发表时间:
2006-10-01
影响因子:
1.8
通讯作者:
Matsuhashi, Shinpei
Matsuhashi, Shinpei
中科院分区:
工程技术3区
文献类型:
--
作者:
Kawachi, Naoki;Sakamoto, Koichi;Matsuhashi, Shinpei

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正电子发射示踪成像系统(PETIS)和碳-11标记的二氧化碳((CO2)-C-11)可用于植物叶片光合作用过程的成像。此外,(11)C动力学有助于光合作用生理功能参数的估计。PETIS测量在四种光照条件下进行,每次将一片叶子暴露于(CO2)-C-11气体。为了估计光合作用参数的速率常数,输入(11)CO2气体和叶响应的时间-活性曲线拟合到适当的房室示踪动力学模型,认为光合作用和蔗糖输出速率常数作为流入和流出,分别。通过这种方法获得的数据显示出合理的响应相对于叶片的光环境,它们是重要的,讨论光合作用与植物生理学和农业。
The positron emitting tracer imaging system (PETIS) and carbon-11-labeled carbon dioxide ((CO2)-C-11) can be used for imaging the photosynthesis process in plant leaves. Further, (11) C kinetics facilitate the estimation of the physiological function parameters of photosynthesis. PETIS measurements were performed under four light conditions for each exposure of a single leaf to (CO2)-C-11 gas. In order to estimate the rate constants of the photosynthesis parameters, the time-activity curves of the input (11) CO2 gas and the leaf response were fitted to an appropriate compartmental tracer kinetic model that considers photoassimilation and sucrose export rate constants as influx and efflux, respectively. The data obtained by this method show a reasonable response with respect to the photoenvironment of the leaf, and they are important for discussing photosynthesis with regard to plant physiology and agriculture.