A 125-BASE-PAIR CEN6 DNA FRAGMENT IS SUFFICIENT FOR COMPLETE MEIOTIC AND MITOTIC CENTROMERE FUNCTIONS IN SACCHAROMYCES-CEREVISIAE
A 125-BASE-PAIR CEN6 DNA FRAGMENT IS SUFFICIENT FOR COMPLETE MEIOTIC AND MITOTIC CENTROMERE FUNCTIONS IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.9.8.3342
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发表时间：
1989-08-01
影响因子：
5.3
通讯作者：
HEGEMANN, JH
中科院分区：
文献类型：
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作者：
COTTAREL, G;SHERO, JH;HEGEMANN, JH
酿酒酵母着丝粒含有一个长度为111至119个碱基对(bp)的保守区域，其特征在于三个保守的DNA元件CDEI、CDEII和CDEIII。我们分离了仅包含这些保守元件的 125 bp CEN6 DNA 片段（命名为 ML CEN6），并对其在环状微型染色体和大线性染色体片段上与染色体背景完全分离进行了分析。结果表明，这个 125 bp CEN6 DNA 片段本身足以完成有丝分裂和减数分裂着丝粒功能。
Saccharomyces cerevisiae centromeres contain a conserved region ranging from 111 to 119 base pairs (bp) in length, which is characterized by the three conserved DNA elements CDEI, CDEII, And CDEIII. We isolated a 125-bp CEN6 DNA fragment (named ML CEN6) containing only these conserved elements and assayed it completely separated from its chromosomal context on circular minichromosomes and on a large linear chromosome fragment. The results show that this 125-bp CEN6 DNA fragment is by itself sufficient for complete mitotic and meiotic centromere functions.