Consistent inhibition of HIV-1 replication in CD4+ T cells by acyclovir without detection of human herpesviruses.
Consistent inhibition of HIV-1 replication in CD4+ T cells by acyclovir without detection of human herpesviruses.
复制标题
阿昔洛韦持续抑制 CD4 T 细胞中的 HIV-1 复制，且未检测到人类疱疹病毒。
DOI：
10.1128/jvi.02423-10
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发表时间：
2011
影响因子：
5.4
通讯作者：
Siliciano,RobertF
中科院分区：
文献类型：
--
作者：
McMahon,MoiraA;Parsons,TeresaL;Shen,Lin;Siliciano,JanetD;Siliciano,RobertF
阿昔洛韦是一种核苷类似物，被认为对人类疱疹病毒具有特异性，因为它需要病毒编码的酶将其磷酸化为单磷酸阿昔洛韦。最近，三磷酸阿昔洛韦被证明是人类免疫缺陷病毒1型（HIV-1）逆转录酶的直接抑制剂。在这里，我们表明，阿昔洛韦是一种抑制HIV-1复制的CD 4 +T细胞从脐带血，有检测不到的水平的八种人类疱疹病毒。此外，通过反相高效液相色谱法（RP-HPLC）检测阿昔洛韦磷酸盐，并在脐带血的引物延伸试验中进行定量。数据支持无环鸟苷作为疱疹病毒阴性细胞中HIV-1复制的抑制剂。
Acyclovir, a nucleoside analog, is thought to be specific for the human herpesviruses because it requires a virally encoded enzyme to phosphorylate it to acyclovir monophosphate. Recently, acyclovir triphosphate was shown to be a direct inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase. Here, we showed that acyclovir is an inhibitor of HIV-1 replication in CD4+T cells from cord blood that have undetectable levels of the eight human herpesviruses. Additionally, acyclovir phosphates were detected by reverse-phase-high performance liquid chromatography (RP-HPLC) and quantified in a primer extension assay from cord blood. The data support acyclovir as an inhibitor of HIV-1 replication in herpesvirus-negative cells.