Antiviral effect of PmRab7 knock-down on inhibition of Laem-Singh virus replication in black tiger shrimp.
Antiviral effect of PmRab7 knock-down on inhibition of Laem-Singh virus replication in black tiger shrimp.
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DOI：
10.1016/j.antiviral.2010.06.013
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发表时间：
2010-10
影响因子：
7.6
通讯作者：
Chalermporn Ongvarrasopone;Ekapol Chomchay;S. Panyim
中科院分区：
文献类型：
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作者：
Chalermporn Ongvarrasopone;Ekapol Chomchay;S. Panyim
PmRab7是斑节对虾的一种小GTP酶蛋白，可能参与多种对虾病毒的复制。本研究利用针对PmRab7基因的双链RNA(dsRNA-PmRab7)进行RNA干扰(RNAi)，以沉默PmRab7的表达，以研究其对Laem-Singh病毒(LSNV)复制的抑制作用。病毒攻击前24小时注射dsRNA-PmRab7后，攻击后3天PmRab7基因表达显著下降，LSNV复制受到完全抑制。在治疗模式中，在LSNV攻击后第1天注射dsRNA-PmRab7，但在第3天或第5天不注射dsRNA-PmRab7，可抑制LSNV的复制。这些结果为利用dsRNA-PmRab7防治对虾LSNV感染奠定了基础。
PmRab7 is a Penaeus monodon small GTPase protein possibly involved in replication of several shrimp viruses. In this study RNA interference (RNAi) using double-stranded RNA (dsRNA) targeting PmRab7 gene (dsRNA-PmRab7) was employed to silence the expression of PmRab7 to investigate the inhibitory effect on Laem-Singh virus (LSNV) replication. Injection of dsRNA-PmRab7 24h before challenge with the virus resulted in a drastic decrease of PmRab7 mRNA and complete inhibition of LSNV replication at 3 days post-challenge. In a therapeutic mode, shrimp injected with dsRNA-PmRab7 1 day but not at 3 or 5 days post-LSNV challenge resulted in inhibition of LSNV replication. These results pave the way to use dsRNA-PmRab7 to prevent or cure LSNV infection in shrimp.