ANCA patients have T cells responsive to complementary PR-3 antigen.
ANCA patients have T cells responsive to complementary PR-3 antigen.
复制标题
ANCA 患者具有对互补 PR-3 抗原有反应的 T 细胞。
DOI：
10.1038/ki.2008.309
复制
发表时间：
2008
影响因子：
19.6
通讯作者：
Preston,GloriaA
中科院分区：
文献类型：
--
作者：
Yang,Jiajin;Bautz,DavidJ;Lionaki,Sofia;Hogan,SusanL;Chin,Hyunsook;Tisch,RolandM;Schmitz,JohnL;Pressler,BarrakM;Jennette,JCharles;Falk,RonaldJ;Preston,GloriaA
Some patients with proteinase 3 specific anti-neutrophil cytoplasmic autoantibodies (PR3-ANCA) also have antibodies that react to complementary-PR3 (cPR3), a protein encoded by the antisense RNA of the PR3 gene. To study whether patients with anti-cPR3 antibodies have cPR3-responsive memory T cells we selected conditions that allowed cultivation of memory cells but not naïve cells. About half of the patients were found to have CD4+TH1 memory cells responsive to the cPR3138–169-peptide; while only a third of the patients had HI-PR3 protein responsive T cells. A significant number of T cells from patients responded to cPR3138–169peptide and to HI-PR3 protein by proliferation and/or secretion of IFN-γ, compared to healthy controls while there was no response to scrambled peptide. Cells responsive to cPR3138–169-peptide were not detected in MPO-ANCA patients suggesting that this response is specific. The HLADRB1* 15 allele was significantly overrepresented in our patient group and is predicted to bind cPR3138–169peptide with high affinity. Regression analysis showed a significant likelihood that anti-cPR3 antibodies and cPR3-specific T cells coexist in individuals, consistent with an immunological history of encounter with a PR3-complementary protein. We suggest that the presence of cells reacting to potential complementary protein pairs might provide an alternative mechanism for auto-immune diseases.
