Fabrication of biopolymeric complex coacervation core micelles for efficient tea polyphenol delivery via a green process.
Fabrication of biopolymeric complex coacervation core micelles for efficient tea polyphenol delivery via a green process.
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DOI：
10.1021/la303062j
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发表时间：
2012-10
期刊：
影响因子：
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通讯作者：
Huihui Zhou;Xiaoyi Sun;Lili Zhang;Pei Zhang;Juan Li;Younian Liu
中科院分区：
文献类型：
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作者：
Huihui Zhou;Xiaoyi Sun;Lili Zhang;Pei Zhang;Juan Li;Younian Liu
纳米胶囊化技术是提高茶多酚生物利用度的一种有效方法。本研究采用绿色工艺，以生物高分子为载体，制备了一种新型的复合凝聚核心胶束（C3 Ms），并用于茶多酚的高效释放。首先，利用美拉德反应合成明胶-葡聚糖缀合物。然后通过将明胶-葡聚糖缀合物与TPP混合来制备C3 Ms。研究了影响C3 Ms自组装的各种因素。在最佳条件下，得到的C3 Ms为纳米级，平均粒径为86 nm，分布较窄。C3 Ms的形成是由于疏水作用和氢键作用而不是静电作用。透射电子显微镜（TEM）和扫描电子显微镜（SEM）结果表明，C3 Ms为球形，具有核壳结构。电位测量表明，核由具有TPP的明胶组成，而壳由葡聚糖片段组成。C3 Ms的包封效率与pH无关，但负载能力是可控的，并且高达360wt%（重量/蛋白质重量）。此外，C3 Ms在体外显示TPP的持续释放。MTT法显示，C3 Ms对MCF-7细胞的细胞毒性与游离TPP相当甚至更强。
Nanoencapsulation is a promising method to improve the bioavailability of tea polyphenol (TPP). In this work, we adopted a green process to develop a new kind of complex coacervation core micelles (C3Ms) based on biopolymers for efficient tea polyphenol delivery. First, gelatin-dextran conjugate was synthesized using Maillard reaction. Then the C3Ms were produced by mixing gelatin-dextran conjugate with TPP. Variable factors on the self-assembly of the C3Ms were investigated. Under optimal conditions, the obtained C3Ms are of nanosize (average 86 nm in diameter) with narrow distribution. The formation of the C3Ms is attributed to hydrophobic interaction and hydrogen bonding instead of electrostatic interaction. Transmission electron microscope (TEM) and scanning electron microscope (SEM) results showed that C3Ms have a spherical shape with core-shell structure. ζ-Potential measurement suggested that the core is composed of gelatin with TPP, whereas the shell is composed of dextran segments. The encapsulation efficiency of the C3Ms is pH-independent, but the loading capacity is controllable and as high as 360 wt % (weight/weight of protein). In addition, the C3Ms show sustained release of TPP in vitro. MTT assay revealed that the C3Ms have comparable or even stronger cytotoxicity against MCF-7 cells than free TPP.