The role of microtubule dynamics in growth cone motility and axonal growth.
The role of microtubule dynamics in growth cone motility and axonal growth.
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DOI：
10.1083/jcb.128.1.139
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发表时间：
1995-01
期刊：
影响因子：
--
通讯作者：
Kirschner MW
中科院分区：
文献类型：
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作者：
Tanaka E;Ho T;Kirschner MW
生长锥包含动态和相对稳定的微管群体，其在运动和轴突生长中的功能是未知的。我们已经使用长春碱在低剂量下抑制微管动力学而没有明显的解聚，以探测这些动力学在生长锥行为中的作用。在仅干扰动力学的长春碱剂量下，生长锥的向前和持续运动受到抑制，生长锥徘徊而没有明显的向前移位;在长春碱被冲洗后，生长锥迅速恢复向前生长。在这些神经元中的荧光标记的微管的直接可视化显示，在没有动态微管的情况下，剩余的聚合物质量不侵入外周板层，并且不经历通常的成束和张开的循环，并且生长锥停止向前运动。这些实验证明了动态微管在生长锥中允许微管重排的作用。这些重排似乎是必要的微管捆绑，随后的合并周围的束皮层形成新的轴突，和向前易位的生长锥。
The growth cone contains dynamic and relatively stable microtubule populations, whose function in motility and axonal growth is uncharacterized. We have used vinblastine at low doses to inhibit microtubule dynamics without appreciable depolymerization to probe the role of these dynamics in growth cone behavior. At doses of vinblastine that interfere only with dynamics, the forward and persistent movement of the growth cone is inhibited and the growth cone wanders without appreciable forward translocation; it quickly resumes forward growth after the vinblastine is washed out. Direct visualization of fluorescently tagged microtubules in these neurons shows that in the absence of dynamic microtubules, the remaining mass of polymer does not invade the peripheral lamella and does not undergo the usual cycle of bundling and splaying and the growth cone stops forward movement. These experiments argue for a role for dynamic microtubules in allowing microtubule rearrangements in the growth cone. These rearrangements seem to be necessary for microtubule bundling, the subsequent coalescence of the cortex around the bundle to form new axon, and forward translocation of the growth cone.