Sensitivity of the human circadian system to short-wavelength (420-nm) light
Sensitivity of the human circadian system to short-wavelength (420-nm) light
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DOI：
10.1177/0748730408323089
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发表时间：
2008-10-01
影响因子：
3.5
通讯作者：
Rollag, Mark D.
中科院分区：
文献类型：
--
作者：
Brainard, George C.;Sliney, David;Rollag, Mark D.
光的昼夜节律和神经行为效应主要是由哺乳动物眼睛中含有光色素黑素的视网膜神经节细胞光感受器介导的。使用啮齿动物、猴子和人类对这些反应进行的九项行动光谱研究表明，在可见光谱的蓝色区域，峰值灵敏度在459到484 nm之间，但光谱的短波灵敏度存在一些不一致。这项工作的目的是量化人类志愿者对抑制血浆褪黑素的单色420 nm光的敏感性。成年女性(n=14)和男性(n=12)受试者参与了两项研究，每项研究均采用受试者内设计。在一项频率响应研究中，受试者(n=8)接受了8次420 nm的光辐射测试，范围在10(10)到10(14)光子/厘米(2)/秒的4对数单位光子密度范围内，并进行了1次暗对照飞行。在另一项研究中，受试者(n=18)完成了一项实验，比较了褪黑素抑制和同等光子剂量(1.21。X 10(13)光子/厘米(2)/秒)420 nm和460 nm单色光和暗曝光控制夜间。第一项研究表明，420nm光和褪黑素抑制(p<；0.001)之间存在明显的通量-响应关系，半饱和常数为2.74x10(11)光子/厘米(2)/秒。第二项研究表明，460 nm的光比420 nm的光抑制褪黑素的作用要强得多(p<；0.04)。总之，这些结果阐明了人类褪黑素抑制作用光谱的可见短波敏感度。这一基本的生理学发现可能有助于优化治疗和其他应用的照明。
The circadian and neurobehavioral effects of light are primarily mediated by a retinal ganglion cell photoreceptor in the mammalian eye containing the photopigment melanopsin. Nine action spectrum studies using rodents, monkeys, and humans for these responses indicate peak sensitivities in the blue region of the visible specturm ranging from 459 to 484 nm, with some disagreement in short-wavelength sensitivity of the spectrum. The aim of this work was to quantify the sensitivity of human volunteers to monochromatic 420-nm light for plasma melatonin suppression. Adult female (n = 14) and male (n = 12) subjects participated in 2 studies, each employing a within-subjects design. In a ftuence-response study, subjects (n = 8) were tested with 8 light irradiances at 420 nm ranging over a 4-log unit photon density range of 10(10) to 10(14) photons/cm(2)/sec and 1 dark control flight. In the other study subjects (n = 18) completed an experiment comparing melatonin suppression with equal photon doses (1.21. X 10(13) photons/cm(2)/sec) of 420 nm ans 460 nm monochromatic light and a dark exposure control night. The first study demonstrated a clear fluence-response relationship between 420-nm light and melatonin suppression (p < 0.001) with a half-saturation constant of 2.74 x 10(11) photons/cm(2)/sec. The second study showed that 460-nm light is significantly stronger than 420-nm light for suppressing melatonin (p < 0.04). Together, the results clarify the visible short-wavelenght sensitivity of the human melatonin suppression action spectrum. This basic physiological finding may be useful for optimizing lighting for therapeutic and other applications.