EUS-guided photodynamic therapy with verteporfin for ablation of normal pancreatic tissue: a pilot study in a porcine model (with video)
EUS-guided photodynamic therapy with verteporfin for ablation of normal pancreatic tissue: a pilot study in a porcine model (with video)
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DOI：
10.1016/j.gie.2007.08.020
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发表时间：
2008-05-01
影响因子：
7.7
通讯作者：
Brugge, William R.
中科院分区：
文献类型：
--
作者：
Yusuf, Tony E.;Matthes, Kai;Brugge, William R.
背景：使用光敏剂卟啉钠进行 EUS 引导的光动力疗法 (PDT) 已被证明可有效消融胰腺组织。 目的：本研究的目的是确定使用维替泊芬（一种光敏性较低的光敏剂）进行 EUS PDT 的有效性和安全性。设计：对在 EUS 前静脉注射 6 mg/m2 维替泊芬的 6 头猪进行前瞻性研究。医院动物实验室。干预措施：用 EUS 定位胰腺尾部，并用于引导光导管的放置。胰尾暴露于激光（689 nm）10、15或20分钟。主要结果测量：进行后续腹部CT、肝肾功能测试以及血清胰酶水平。在尸检程序后获得7粘土的胰腺组织学结果：在所有动物中都观察到胰尾内的局部组织坏死（直径范围6.6-30.5毫米）。坏死组织的直径与光线的接近程度直接相关。未观察到术后并发症。 局限性：正常动物模型（缺乏恶性组织）。结论：EUS引导下用维替泊芬对猪胰腺进行PDT，以剂量相关的方式实现了局部胰腺组织消融。
Background: EUS-guided photodynamic therapy (PDT) with the photosensitizing agent porfimer sodium has been shown to he effective in ablation of pancreatic tissue.Objective: The objective of this study was to determine the effectiveness and safety of EUS PDT with verteporfin, a photosensitizer associated with less photosensitivity.Design: Prospective investigation of 6 swine that received an IV injection of 6 mg/m2 of verteporfin before EUS.Setting: Hospital animal laboratory.Interventions: The tail of the pancreas was located with EUS and was used to guide the placement of a light catheter. The pancreatic tail was exposed to 10, 15, or 20 minutes of laser light (689 nm).Main Outcome Measurements: Follow-up abdominal CT, liver and renal function tests, and serum pancreatic enzymes levels were performed. Histology of the pancreas was obtained 7 clays after the procedure on necropsyResults: Localized tissue necrosis within the pancreatic tail (range 6.6-30.5 mm in diameter) was seen in all animals. The diameter of the necrotic tissue was directly related to the close of light. No post-procedural complications were observed.Limitations: Normal animal model (lack of malignant tissue).Conclusions: EUS-guided PDT of porcine pancreas with verteporfin achieved localized pancreatic tissue ablation in a dose-related fashion.