Inhibition of inflammatory reactions in 2,4-Dinitrochlorobenzene induced Nc/Nga atopic dermatitis mice by non-thermal plasma.
Inhibition of inflammatory reactions in 2,4-Dinitrochlorobenzene induced Nc/Nga atopic dermatitis mice by non-thermal plasma.
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DOI：
10.1038/srep27376
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发表时间：
2016-06-08
影响因子：
4.6
通讯作者：
Kim GC
中科院分区：
文献类型：
--
作者：
Choi JH;Song YS;Lee HJ;Hong JW;Kim GC
非热等离子体（NTP）最近被介绍和报道为一种具有一系列医学和生物学作用的新工具。虽然已经进行了许多使用NTP的研究，但尚未调查NTP与免疫反应之间的直接关系。特别是，NTP对特应性皮炎（AD）的影响尚未探讨。在这里，测试NTP是否控制AD的免疫反应。采用NTP处理促炎细胞因子刺激的角质形成细胞和DNCB（2,4-二硝基氯苯）诱导的特应性皮炎小鼠，观察细胞和皮肤组织的免疫反应。经NTP处理的细胞CCL11、CCL13、CCL17表达水平降低，NF-κB活性下调。对ad诱导的小鼠反复给予NTP可降低肥大细胞和嗜酸性粒细胞数量、IgE、CCL17、IFNγ水平，并抑制皮肤病变中NF-κB活性。此外，NTP和1%氢化可的松乳膏的联合治疗显著降低了AD的免疫反应，而不是单独使用这两种治疗。综上所述，本研究表明NTP通过调节NF-κB活性显著抑制AD的几种免疫反应。因此，NTP可用于抑制AD等严重皮肤炎症性疾病的过度免疫反应。
Non-thermal plasma (NTP) has recently been introduced and reported as a novel tool with a range of medicinal and biological roles. Although many studies using NTP have been performed, none has investigated the direct relationship between NTP and immune responses yet. Especially, the effects of NTP on atopic dermatitis (AD) were not been explored. Here, NTP was tested whether it controls immune reactions of AD. NTP treatment was administered to pro-inflammatory cytokine-stimulated keratinocytes and DNCB (2,4-Dinitrochlorobenzene)-induced atopic dermatitis mice, then the immune reactions of cells and skin tissues were monitored. Cells treated with NTP showed decreased expression levels of CCL11, CCL13, and CCL17 along with down-regulation of NF-κB activity. Repeated administration of NTP to AD-induced mice reduced the numbers of mast cells and eosinophils, IgE, CCL17, IFNγ levels, and inhibited NF-κB activity in the skin lesion. Furthermore, combined treatment with NTP and 1% hydrocortisone cream significantly decreased the immune responses of AD than that with either of these two treatments individually. Overall, this study revealed that NTP significantly inhibits several immune reactions of AD by regulating NF-κB activity. Therefore, NTP could be useful to suppress the exaggerated immune reactions in severe skin inflammatory diseases such as AD.