The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry.
The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry.
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DOI：
10.1016/j.jdsr.2016.09.001
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发表时间：
2017-05
期刊：
影响因子：
--
通讯作者：
Egusa H
中科院分区：
文献类型：
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作者：
Niibe K;Zhang M;Nakazawa K;Morikawa S;Nakagawa T;Matsuzaki Y;Egusa H
牙周组织缺损的有效再生治疗最近已被证明使用间充质基质/干细胞（MSC）。此外，目前的生物工程技术已经能够在小鼠中从头制造牙周牙单元。这些尖端技术有望解决再生牙科领域未满足的需求。然而，为了实现有效和稳定的治疗结果，制备适当的干细胞来源是必不可少的。许多研究人员正在研究成体干细胞在再生牙科中的应用;骨髓源性间充质干细胞（BM-MSCs）特别有前途，目前已用于临床。然而，目前的BM-MSC分离技术导致异质的、不可再现的细胞群，因为缺乏鉴定的不同BM-MSC表面标记。最近，在小鼠（PDGFRα+和Sca-1+）和人（LNGFR+、THY-1+和VCAM-1+）中报告了BM-MSC的细胞表面标志物的特定子集，促进了独特富集的BM-MSC（所谓的“纯化MSC”）的分离。值得注意的是，富集的BM-MSC群体含有神经嵴衍生的细胞，其可以分化成神经嵴和间充质谱系的细胞。在这篇综述中，富集的BM-MSCs的特性概述了其在未来再生牙科中的潜在应用。
Effective regenerative treatments for periodontal tissue defects have recently been demonstrated using mesenchymal stromal/stem cells (MSCs). Furthermore, current bioengineering techniques have enabled de novo fabrication of tooth-perio dental units in mice. These cutting-edge technologies are expected to address unmet needs within regenerative dentistry. However, to achieve efficient and stable treatment outcomes, preparation of an appropriate stem cell source is essential. Many researchers are investigating the use of adult stem cells for regenerative dentistry; bone marrow-derived MSCs (BM-MSCs) are particularly promising and presently used clinically. However, current BM-MSC isolation techniques result in a heterogeneous, non-reproducible cell population because of a lack of identified distinct BM-MSC surface markers. Recently, specific subsets of cell surface markers for BM-MSCs have been reported in mice (PDGFRα+ and Sca-1+) and humans (LNGFR+, THY-1+ and VCAM-1+), facilitating the isolation of unique enriched BM-MSCs (so-called “purified MSCs”). Notably, the enriched BM-MSC population contains neural crest-derived cells, which can differentiate into cells of neural crest- and mesenchymal lineages. In this review, characteristics of the enriched BM-MSCs are outlined with a focus on their potential application within future regenerative dentistry.