Ecdysone receptor isoform‐B mediates soluble trehalase expression to regulate growth and development in the mirid bug, Apolygus lucorum (Meyer‐Dür)
Ecdysone receptor isoform‐B mediates soluble trehalase expression to regulate growth and development in the mirid bug, Apolygus lucorum (Meyer‐Dür)
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DOI：
10.1111/imb.12185
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发表时间：
2015-12
影响因子：
2.6
通讯作者：
Y-A Tan;L-B Xiao;J. Zhao;Y-F Xiao;Y. Sun;L. Bai
中科院分区：
文献类型：
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作者：
Y-A Tan;L-B Xiao;J. Zhao;Y-F Xiao;Y. Sun;L. Bai
蜕皮激素受体（Ecdysone receptor，EcR）是蜕皮激素的激素受体，严格调控昆虫的生长发育。然而，EcR的作用机制并不十分清楚。本研究克隆了绿盲蝽EcR亚型B（AlEcR B）的cDNA，并对其表达谱进行了研究。我们在体内使用系统性RNA干扰降低AlEcR B mRNA表达，并获得敲除标本。对这些样本的检查表明，AlEcR B是若虫存活所必需的，并且表达减少与发育时间延长和若虫重量降低相关。为了研究所观察到的抑制作用的潜在分子机制，我们选择海藻糖酶进行详细的研究。编码可溶性海藻糖酶（AlTre-1）的转录物被20-羟基蜕皮激素上调，并与AlEcR-B的mRNA表达一致。与对照组相比，B敲低表达后，存活的果蝇中肠中AlTre-1的表达谱、可溶性海藻糖酶活性和翻译蛋白水平均下调。相比之下，膜结合海藻糖酶活性、相关基因表达和翻译蛋白水平保持在其初始水平。但在相同条件下，海藻糖含量显著增加，葡萄糖含量显著降低。我们推测B可能通过调节AlTre-1的表达来调控A. lucorum，其为B的功能提供了扩展信息。
Ecdysone receptor (EcR) is the hormonal receptor of ecdysteroids and strictly regulates growth and development in insects. However, the action mechanism of EcR is not very clear. In this study, the cDNA of EcR isoform‐B was cloned from Apolygus lucorum (AlEcR‐B) and its expression profile was investigated. We reduced AlEcR‐B mRNA expression using systemic RNA interference in vivo, and obtained knockdown specimens. Examination of these specimens indicated that AlEcR‐B is required for nymphal survival, and that reduced expression is associated with longer development time and lower nymphal weight. To investigate the underlying molecular mechanism of the observed suppression effects, we selected trehalase for a detailed study. Transcript encoding soluble trehalase (AlTre‐1) was up‐regulated by 20‐hydroxyecdysone and in agreement with the mRNA expression of AlEcR‐B. The expression profile of AlTre‐1, soluble trehalase activity and translated protein level in the midgut of surviving nymphs were down‐regulated, compared with controls, after the knockdown expression of AlEcR‐B. By contrast, membrane‐bound trehalase activity, the related gene expression and translated protein level remained at their initial levels. However, trehalose content significantly increased and the glucose content significantly decreased under the same conditions. We propose that AlEcR‐B controls normal carbohydrate metabolism by mediating the expression of AlTre‐1 to regulate the growth and development in A. lucorum, which provide an extended information into the functions of AlEcR‐B.