A Simple, Sensitive Spectrophotometric Assay for Extrinsic (Tissue-Type) Plasminogen Activator Applicable to Measurements in Plasma
A Simple, Sensitive Spectrophotometric Assay for Extrinsic (Tissue-Type) Plasminogen Activator Applicable to Measurements in Plasma
复制标题
一种简单、灵敏的分光光度法测定外源性（组织型）纤溶酶原激活剂，适用于血浆测量
DOI：
--
复制
发表时间：
1982
影响因子：
6.7
通讯作者：
G. Wijngaards
中科院分区：
文献类型：
--
作者：
J. Verheijen;E. Mullaart;G. Chang;C. Kluft;G. Wijngaards
在Drapier等人的抛物线法的基础上，设计了一种外源性纤溶酶原激活剂的间接分光光度法。该系统包含激活剂、纤溶酶原、合成纤溶酶底物H-D-Val-Leu-Lys-pNA （S-2251, Kabi）和用溴化氰处理纤维蛋白原制备的可溶性纤维蛋白原片段混合物。由于外源性纤溶酶原激活剂对纤溶酶原活化的特异性刺激，这些纤维蛋白原片段的加入大大提高了方法的敏感性和特异性。对测定条件进行了优化，并证明了外源性纤溶酶原激活剂测定血浆优球蛋白组分的应用。
Summary An indirect spectrophotometric assay for extrinsic plasminogen activator has been devised, which is based on the parabolic assay of Drapier et al. (5). The system contains activator, plasminogen, the synthetic plasmin substrate H-D-Val-Leu-Lys-pNA (S-2251, Kabi) and a mixture of soluble fibrinogen fragments prepared by treatment of fibrinogen with cyanogen bromide. The addition of these fibrinogen fragments considerably enhances the sensitivity and specificity of the method owing to specific stimulation of the plasminogen activation by extrinsic plasminogen activator. The assay conditions were optimized and the application for extrinsic plasminogen activator measurements in plasma euglobulin fractions is demonstrated.