Local Expression of Vaginal Th1 and Th2 Cytokines in Murine Vaginal Candidiasis under Different Immunity Conditions
Local Expression of Vaginal Th1 and Th2 Cytokines in Murine Vaginal Candidiasis under Different Immunity Conditions
复制标题
DOI：
10.1007/s11596-008-0423-z
复制
发表时间：
2008-08-01
影响因子：
--
通讯作者：
Li, Jiawen
中科院分区：
文献类型：
--
作者：
Chen, Shanjuan;Li, Shaohua;Li, Jiawen
为了研究不同免疫条件下实验性阴道念珠菌病大鼠阴道Th1和Th2细胞因子的表达，我们建立了ICR小鼠阴道念珠菌病模型和雌激素处理小鼠阴道念珠菌病免疫抑制模型。未接受雌激素治疗的小鼠作为对照。采用RT-PCR方法检测小鼠阴道组织中Th1 (IL-2)/Th2 （IL-4、IL-10、tgf - β 1）细胞因子mRNA表达水平。正常免疫条件下，局部组织细胞数均有不同程度的增加。IL-2 mRNA在感染早期升高，IL-10 mRNA在感染晚期短暂升高。tgf - β 1的产生持续增加。同时，免疫抑制组感染期间IL-2 mRNA表达受到抑制，IL-4、IL-10、tgf - β 1水平均不同程度高于正常免疫组。感染早期高水平的IL-2 mRNA与粘膜白色念珠菌（C. albicans）的清除有关，其表达抑制导致免疫抑制粘膜白色念珠菌的清除减少。过表达IL-4和IL-10可显著增强小鼠对白色念珠菌感染的易感性。
To investigate the expression of vaginal Th1 and Th2 cytokines in rats with experimental vaginal candidiasis under different immune conditions, ICR murine vaginal candidiasis model was established and immno-suppressed murine models of vaginal cadidiasis were established in estrogen-treated mice. Non-estrogen-treated mice were used as controls. The mRNA level of Th1 (IL-2)/Th2 (IL-4, IL-10, TGF-beta 1) cytokines in murine vaginal tissues was determined by RT-PCR. The cykotine in local tissues was increased to different extent under normal immune condition. IL-2 mRNA was increased during early stage of infection, while IL-10 was increased transiently during late stage of infection. TGF-beta 1 production was found to be increased persistently. At same time, the expression of IL-2 mRNA was suppressed in immno-suppressed group, and the level of IL-4, IL-10, and TGF-beta 1 were higher than the normal immunity group to different degree during infection. The high level of IL-2 mRNA during early stage of infection was associated with clearance of mucosal Candidia albicans (C. albicans), and its expression suppressed leading to decreased clearance of mucosal C. albican in immuno-suppression. The over-expression of IL-4 and IL-10 could significantly enhance the susceptibility to C. albicans infection in mice.