Fragrance of the rice grain achieved via artificial microRNA-induced down-regulation of OsBADH2
Fragrance of the rice grain achieved via artificial microRNA-induced down-regulation of OsBADH2
复制标题
通过人工 microRNA 诱导 OsBADH2 下调实现米粒香味
DOI：
10.1111/j.1439-0523.2012.01989.x
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发表时间：
2012-10-01
期刊：
影响因子：
2
通讯作者：
Hu, Peisong
中科院分区：
文献类型：
--
作者：
Chen, Mingliang;Wei, Xiangjin;Hu, Peisong
摘要2-乙酰基-1-吡咯啉（2AP）是稻米中产生香味的主要化合物。在香米品种中，BADH 2（甜菜碱醛脱氢酶2）被灭活。在这里，我们描述了针对BADH 2的amiRNA（人工microRNA）转基因在水稻中的效果。在amiRNA由玉米泛素启动子驱动的转基因株系中，BADH 2（而非BADH 1）表达下调，籽粒2AP含量也显著升高;而野生型株系籽粒中未检测到2AP。转基因植株叶片脯氨酸含量的增加是通过同时下调PRODH和上调P5 CS来实现的。在转基因株系中，相同的amiRNA由胚乳特异性启动子GluC驱动，对BADH 2表达没有影响，并且谷物2AP的含量与野生型谷物中的含量没有差异。
With 6 figures and 1 table Abstract 2-acetyl-1-pyrroline (2AP) is the principal compound responsible for grain fragrance in rice. In fragrant rice cultivars, BADH2 (betaine-aldehyde dehydrogenase 2) is inactivated. Here, we describe the effect of amiRNA (artificial microRNA) transgenesis targeted at BADH2 in rice. BADH2 (but not BADH1) expression was down-regulated in transgenic lines where the amiRNA was driven by the maize ubiquitin promoter, and in these lines, grain 2AP content was also significantly elevated; meanwhile, 2AP could not be detected in the grain of wild-type lines. The leaf proline content in the transgenics was increased through the simultaneous down-regulation of PRODH and up-regulation of P5CS. In transgenic lines where the same amiRNA was driven by the endosperm-specific promoter GluC, no effect on BADH2 expression was detectable, and the content of grain 2AP did not differ from that present in wild-type grains.